[Influence of luxS of Streptococcus mutans on biofilm of dental plaque] Export

@article{citeulike:5916114, abstract = {OBJECTIVE: To construct luxS mutant aften luxS gene of Streptococcus mutans (S. mutans) was knocked out, and examine their ability of biofilm formation. METHODS: A recombinant plasmid containing the flanking fragment of luxS of S. mutans was transformed into S. mutans UA159, and selected by brain heart infusion (BHI) agar medium with kanamicin. The luxS mutant further confirmed via polymerase chain reaction (PCR) and the autoinducer-2 (AI-2) bioluminescence assay of Vibrio harveyi (V. harveyi), and ability of luxS mutant and S. mutans UA159 biofilm formation was examined in different phases, in BHI medium with 1\% sucrose and 1\% glycose by scanning electron microscopy (SEM). RESULTS: LuxS-deficient S. mutans strains were successfully constructed. Compared with S. mutans UA159, the luxS mutant maintained in BHI medium containing 1\% sucrose displayed an apparent defect in biofilm formation, while they showed no significant deviation in BHI medium containing 1\% glycose. CONCLUSION: luxS gene in S.mutans can play a role in dental plaque biofilm formation, and the luxS gene is possible to regulate sucrose-dependent biofilm formation.}, author = {Tong, Zhong-chun C. and Ni, Long-xing X. and Ma, Li-fang F. and Zhou, Lin and Hou, Bo and Zhao, Xiu}, citeulike-article-id = {5916114}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/19769255}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=19769255}, issn = {1000-1182}, journal = {Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology}, keywords = {biofilm, dental, luxs, mutans, plaque, streptococcus}, month = {August}, number = {4}, pages = {386--389}, posted-at = {2009-10-09 14:33:21}, priority = {2}, title = {[Influence of luxS of Streptococcus mutans on biofilm of dental plaque]}, url = {http://view.ncbi.nlm.nih.gov/pubmed/19769255}, volume = {27}, year = {2009} }

TY - JOUR ID - citeulike:5916114 L3 - citeulike-article-id:5916114 N2 - OBJECTIVE: To construct luxS mutant aften luxS gene of Streptococcus mutans (S. mutans) was knocked out, and examine their ability of biofilm formation. METHODS: A recombinant plasmid containing the flanking fragment of luxS of S. mutans was transformed into S. mutans UA159, and selected by brain heart infusion (BHI) agar medium with kanamicin. The luxS mutant further confirmed via polymerase chain reaction (PCR) and the autoinducer-2 (AI-2) bioluminescence assay of Vibrio harveyi (V. harveyi), and ability of luxS mutant and S. mutans UA159 biofilm formation was examined in different phases, in BHI medium with 1% sucrose and 1% glycose by scanning electron microscopy (SEM). RESULTS: LuxS-deficient S. mutans strains were successfully constructed. Compared with S. mutans UA159, the luxS mutant maintained in BHI medium containing 1% sucrose displayed an apparent defect in biofilm formation, while they showed no significant deviation in BHI medium containing 1% glycose. CONCLUSION: luxS gene in S.mutans can play a role in dental plaque biofilm formation, and the luxS gene is possible to regulate sucrose-dependent biofilm formation. IS - 4 JF - Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology SN - 1000-1182 EP - 389 TI - [Influence of luxS of Streptococcus mutans on biofilm of dental plaque] VL - 27 SP - 386 KW - biofilm KW - dental KW - luxs KW - mutans KW - plaque KW - streptococcus AU - Tong, Zhong-chun AU - Ni, Long-xing AU - Ma, Li-fang AU - Zhou, Lin AU - Hou, Bo AU - Zhao, Xiu PY - 2009/08// UR - http://view.ncbi.nlm.nih.gov/pubmed/19769255 ER -