The nuclear export receptor Xpo1/Crm1 is physically and functionally linked to the spindle pole body in budding yeast Export

@article{citeulike:2933193, abstract = {The spindle pole body (SPB) represents the microtubule organizing center in budding yeast. It is a highly structured organelle embedded in the nuclear membrane which is required to anchor microtubules (MTs) on both sides of the nuclear envelope. The protein Spc72, a component of the SPB, is located at the cytoplasmic face of this organelle and serves as a receptor for the {gamma}-tubulin complex. In this paper we show that it is also a binding partner of the nuclear export receptor Xpo1/Crm1. Xpo1 binds its cargoes in a Ran-dependent fashion via a short leucine-rich nuclear export signal (NES). We show that binding of Spc72 to Xpo1 depends on Ran-GTP and a functional NES in Spc72. Mutations in this NES have severe consequences for mitotic spindle morphology in vivo. This is also the case for xpo1 mutants, which show a reduction in cytoplasmic microtubules. In addition, we find a subpopulation of Xpo1 localized at the spindle pole body. Based on these data, we propose a functional link between Xpo1 and the SPB and discuss a role for this exportin in spindle biogenesis in budding yeast. 10.1128/MCB.02043-07}, author = {Neuber, Anja and Franke, Jacqueline and Wittstruck, Angelika and Schlenstedt, Gabriel and Sommer, Thomas and Stade, Katrin}, citeulike-article-id = {2933193}, citeulike-linkout-0 = {http://dx.doi.org/10.1128/MCB.02043-07}, citeulike-linkout-1 = {http://mcb.asm.org/cgi/content/abstract/28/17/5348}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/18573877}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=18573877}, day = {23}, doi = {10.1128/MCB.02043-07}, journal = {Mol. Cell. Biol.}, keywords = {complex, nuclear, pore, spb, spc72, xpo1crm1}, month = {June}, pages = {MCB.02043-07+}, posted-at = {2008-06-27 10:55:58}, priority = {3}, title = {The nuclear export receptor Xpo1/Crm1 is physically and functionally linked to the spindle pole body in budding yeast}, url = {http://dx.doi.org/10.1128/MCB.02043-07}, year = {2008} }

TY - JOUR ID - citeulike:2933193 L3 - citeulike-article-id:2933193 N2 - The spindle pole body (SPB) represents the microtubule organizing center in budding yeast. It is a highly structured organelle embedded in the nuclear membrane which is required to anchor microtubules (MTs) on both sides of the nuclear envelope. The protein Spc72, a component of the SPB, is located at the cytoplasmic face of this organelle and serves as a receptor for the {gamma}-tubulin complex. In this paper we show that it is also a binding partner of the nuclear export receptor Xpo1/Crm1. Xpo1 binds its cargoes in a Ran-dependent fashion via a short leucine-rich nuclear export signal (NES). We show that binding of Spc72 to Xpo1 depends on Ran-GTP and a functional NES in Spc72. Mutations in this NES have severe consequences for mitotic spindle morphology in vivo. This is also the case for xpo1 mutants, which show a reduction in cytoplasmic microtubules. In addition, we find a subpopulation of Xpo1 localized at the spindle pole body. Based on these data, we propose a functional link between Xpo1 and the SPB and discuss a role for this exportin in spindle biogenesis in budding yeast. 10.1128/MCB.02043-07 JF - Mol. Cell. Biol. TI - The nuclear export receptor Xpo1/Crm1 is physically and functionally linked to the spindle pole body in budding yeast SP - MCB.02043-07 KW - complex KW - nuclear KW - pore KW - spb KW - spc72 KW - xpo1crm1 AU - Neuber, Anja AU - Franke, Jacqueline AU - Wittstruck, Angelika AU - Schlenstedt, Gabriel AU - Sommer, Thomas AU - Stade, Katrin PY - 2008/06/23/ UR - http://dx.doi.org/10.1128/MCB.02043-07 ER -