Cell cycle-dependent kinetochore localization of condensin complex in Saccharomyces cerevisiae Export

@article{citeulike:3037321, abstract = {In budding yeast mitosis is endonuclear and associated with a very limited condensation of the chromosomes. Despite this partial chromosomal condensation, condensin is conserved and essential for the Saccharomyces cerevisiae mitotic cycle. Here, we investigate the localization of condensin during the mitotic cycle. In addition to a constitutive association with rDNA, we have discovered that condensin is localized to the kinetochore in a cell cycle-dependent manner. Shortly after duplication of the spindle pole body, the yeast equivalent of the centrosome, we observed a local enrichment of condensin colocalizing with kinetochore components. This specific association is consistent with mutant phenotypes of chromosome loss and defective sister chromatid separation at anaphase. During a short period of the cell cycle, we observed, at the single cell level, a spatial proximity of condensin and a cohesin rosette, without colocalization. Furthermore, using a genetic screen we demonstrated that condensin localization at kinetochores is specifically impaired in a mutant for ulp2/smt4, an abundant SUMO protease. In conclusion, during chromosome segregation, we established a SUMO-dependent cell cycle-specific condensin concentration colocalizing with kinetochores.}, author = {Bachellier-Bassi, Sophie and Gadal, Olivier and Bourout, Ga\"{e}lle and Nehrbass, Ulf}, citeulike-article-id = {3037321}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.jsb.2008.01.002}, citeulike-linkout-1 = {http://linkinghub.elsevier.com/retrieve/pii/S1047847708000038}, citeulike-linkout-2 = {http://www.sciencedirect.com/science/article/B6WM5-4RJRVMY-2/1/abc6339fad9392d946906e75c2bcfc63}, doi = {10.1016/j.jsb.2008.01.002}, journal = {Journal of Structural Biology}, keywords = {body, cell, cycle, dynamics, kinetochores, pole, scerevisiae, spindle}, month = {May}, number = {2}, pages = {248--259}, posted-at = {2008-07-23 17:27:51}, priority = {2}, title = {Cell cycle-dependent kinetochore localization of condensin complex in Saccharomyces cerevisiae}, url = {http://dx.doi.org/10.1016/j.jsb.2008.01.002}, volume = {162}, year = {2008} }

TY - JOUR ID - citeulike:3037321 L3 - citeulike-article-id:3037321 N2 - In budding yeast mitosis is endonuclear and associated with a very limited condensation of the chromosomes. Despite this partial chromosomal condensation, condensin is conserved and essential for the Saccharomyces cerevisiae mitotic cycle. Here, we investigate the localization of condensin during the mitotic cycle. In addition to a constitutive association with rDNA, we have discovered that condensin is localized to the kinetochore in a cell cycle-dependent manner. Shortly after duplication of the spindle pole body, the yeast equivalent of the centrosome, we observed a local enrichment of condensin colocalizing with kinetochore components. This specific association is consistent with mutant phenotypes of chromosome loss and defective sister chromatid separation at anaphase. During a short period of the cell cycle, we observed, at the single cell level, a spatial proximity of condensin and a cohesin rosette, without colocalization. Furthermore, using a genetic screen we demonstrated that condensin localization at kinetochores is specifically impaired in a mutant for ulp2/smt4, an abundant SUMO protease. In conclusion, during chromosome segregation, we established a SUMO-dependent cell cycle-specific condensin concentration colocalizing with kinetochores. IS - 2 JF - Journal of Structural Biology EP - 259 TI - Cell cycle-dependent kinetochore localization of condensin complex in Saccharomyces cerevisiae VL - 162 SP - 248 KW - body KW - cell KW - cycle KW - dynamics KW - kinetochores KW - pole KW - scerevisiae KW - spindle AU - Bachellier-Bassi, Sophie AU - Gadal, Olivier AU - Bourout, Gaëlle AU - Nehrbass, Ulf PY - 2008/05// UR - http://dx.doi.org/10.1016/j.jsb.2008.01.002 ER -