Defining Molecular Cornerstones during Fibroblast to iPS Cell Reprogramming in Mouse Export

@article{citeulike:2404862, abstract = {Summary Ectopic expression of the transcription factors Oct4, Sox2, c-Myc, and Klf4 in fibroblasts generates induced pluripotent stem (iPS) cells. Little is known about the nature and sequence of molecular events accompanying nuclear reprogramming. Using doxycycline-inducible vectors, we have shown that exogenous factors are required for about 10 days, after which cells enter a self-sustaining pluripotent state. We have identified markers that define cell populations prior to and during this transition period. While downregulation of Thy1 and subsequent upregulation of SSEA-1 occur at early time points, reactivation of endogenous Oct4, Sox2, telomerase, and the silent X chromosome mark late events in the reprogramming process. Cell sorting with these markers allows for a significant enrichment of cells with the potential to become iPS cells. Our results suggest that factor-induced reprogramming is a gradual process with defined intermediate cell populations that contain the majority of cells poised to become iPS cells.}, author = {Stadtfeld, Matthias and Maherali, Nimet and Breault, David T. and Hochedlinger, Konrad}, citeulike-article-id = {2404862}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.stem.2008.02.001}, citeulike-linkout-1 = {http://www.sciencedirect.com/science/article/B8G3V-4RV1YYW-1/2/2078a638aa567aa6d94a383de8a4f381}, doi = {10.1016/j.stem.2008.02.001}, journal = {Cell Stem Cell}, keywords = {ips, mouse, story}, posted-at = {2008-02-21 00:40:28}, priority = {5}, title = {Defining Molecular Cornerstones during Fibroblast to iPS Cell Reprogramming in Mouse}, url = {http://dx.doi.org/10.1016/j.stem.2008.02.001}, volume = {In Press, Corrected Proof} }

TY - JOUR ID - citeulike:2404862 L3 - citeulike-article-id:2404862 N2 - Summary Ectopic expression of the transcription factors Oct4, Sox2, c-Myc, and Klf4 in fibroblasts generates induced pluripotent stem (iPS) cells. Little is known about the nature and sequence of molecular events accompanying nuclear reprogramming. Using doxycycline-inducible vectors, we have shown that exogenous factors are required for about 10 days, after which cells enter a self-sustaining pluripotent state. We have identified markers that define cell populations prior to and during this transition period. While downregulation of Thy1 and subsequent upregulation of SSEA-1 occur at early time points, reactivation of endogenous Oct4, Sox2, telomerase, and the silent X chromosome mark late events in the reprogramming process. Cell sorting with these markers allows for a significant enrichment of cells with the potential to become iPS cells. Our results suggest that factor-induced reprogramming is a gradual process with defined intermediate cell populations that contain the majority of cells poised to become iPS cells. JF - Cell Stem Cell TI - Defining Molecular Cornerstones during Fibroblast to iPS Cell Reprogramming in Mouse VL - In Press, Corrected Proof KW - ips KW - mouse KW - story AU - Stadtfeld, Matthias AU - Maherali, Nimet AU - Breault, David AU - Hochedlinger, Konrad UR - http://dx.doi.org/10.1016/j.stem.2008.02.001 ER -