Green Tea Polyphenol Epigallocatechin-3-gallate Signaling Pathway through 67-kDa Laminin Receptor Export

@article{citeulike:2610147, abstract = {()-Epigallocatechin-3-gallate (EGCG), the principal polyphenol in green tea, has been shown to be a potent chemopreventive agent. Recently, 67-kDa laminin receptor (67LR) has been identified as a cell surface receptor for EGCG that mediates the anticancer activity of EGCG. Indeed, expression of 67LR confers EGCG responsiveness to tumor cells; however, the molecular basis for the anticancer activity of EGCG in vivo is not entirely understood. Here we show that (i) using a direct genetic screen, eukaryotic translation elongation factor 1A (eEF1A) is identified as a component responsible for the anticancer activity of EGCG; (ii) through both eEF1A and 67LR, EGCG induces the dephosphorylation of myosin phosphatase targeting subunit 1 (MYPT1) at Thr-696 and activates myosin phosphatase; and (iii) silencing of 67LR, eEF1A, or MYPT1 in tumor cells results in abrogation of EGCG-induced tumor growth inhibition in vivo. Additionally, we found that eEF1A is up-regulated by EGCG through 67LR. Overall, these findings implicate both eEF1A and MYPT1 in EGCG signaling for cancer prevention through 67LR. 10.1074/jbc.M707892200}, author = {Umeda, Daisuke and Yano, Satomi and Yamada, Koji and Tachibana, Hirofumi}, citeulike-article-id = {2610147}, citeulike-linkout-0 = {http://dx.doi.org/10.1074/jbc.M707892200}, citeulike-linkout-1 = {http://www.jbc.org/cgi/content/abstract/283/6/3050}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/18079119}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=18079119}, day = {8}, doi = {10.1074/jbc.M707892200}, journal = {J. Biol. Chem.}, keywords = {cancer, greentea}, month = {February}, number = {6}, pages = {3050--3058}, posted-at = {2008-03-29 05:48:10}, priority = {2}, title = {Green Tea Polyphenol Epigallocatechin-3-gallate Signaling Pathway through 67-kDa Laminin Receptor}, url = {http://dx.doi.org/10.1074/jbc.M707892200}, volume = {283}, year = {2008} }

TY - JOUR ID - citeulike:2610147 L3 - citeulike-article-id:2610147 N2 - ()-Epigallocatechin-3-gallate (EGCG), the principal polyphenol in green tea, has been shown to be a potent chemopreventive agent. Recently, 67-kDa laminin receptor (67LR) has been identified as a cell surface receptor for EGCG that mediates the anticancer activity of EGCG. Indeed, expression of 67LR confers EGCG responsiveness to tumor cells; however, the molecular basis for the anticancer activity of EGCG in vivo is not entirely understood. Here we show that (i) using a direct genetic screen, eukaryotic translation elongation factor 1A (eEF1A) is identified as a component responsible for the anticancer activity of EGCG; (ii) through both eEF1A and 67LR, EGCG induces the dephosphorylation of myosin phosphatase targeting subunit 1 (MYPT1) at Thr-696 and activates myosin phosphatase; and (iii) silencing of 67LR, eEF1A, or MYPT1 in tumor cells results in abrogation of EGCG-induced tumor growth inhibition in vivo. Additionally, we found that eEF1A is up-regulated by EGCG through 67LR. Overall, these findings implicate both eEF1A and MYPT1 in EGCG signaling for cancer prevention through 67LR. 10.1074/jbc.M707892200 IS - 6 JF - J. Biol. Chem. EP - 3058 TI - Green Tea Polyphenol Epigallocatechin-3-gallate Signaling Pathway through 67-kDa Laminin Receptor VL - 283 SP - 3050 KW - cancer KW - greentea AU - Umeda, Daisuke AU - Yano, Satomi AU - Yamada, Koji AU - Tachibana, Hirofumi PY - 2008/02/08/ UR - http://dx.doi.org/10.1074/jbc.M707892200 ER -