MicroRNA 29c is down-regulated in nasopharyngeal carcinomas, up-regulating mRNAs encoding extracellular matrix proteins Export

@article{citeulike:2630949, abstract = {Using highly sensitive microarray-based procedures, we identified eight microRNAs (miRNAs) showing robust differential expression between 31 laser-capture-microdissected nasopharyngeal carcinomas (NPCs) and 10 normal healthy nasopharyngeal epithelial samples. In particular, miRNA mir-29c was expressed at one-fifth the levels in tumors as in normal epithelium. In NPC tumors, the lower mir-29c levels correlated with higher levels of multiple mRNAs whose 3' UTRs can bind mir-29c at target sequences conserved across many vertebrates. In cultured cells, introduction of mir-29c down-regulated these genes at the level of mRNA and inhibited expression of luciferase encoded by vectors having the 3' UTRs of these genes. Moreover, for each of several genes tested, mutating the mir-29c target sites in the 3' UTR abrogated mir-29c-induced inhibition of luciferase expression. Most of the mir-29c-targeted genes identified encode extracellular matrix proteins, including multiple collagens and laminin {gamma}1, that are associated with tumor cell invasiveness and metastatic potential, prominent characteristics of NPC. Thus, we identify eight miRNAs differentially expressed in NPC and demonstrate the involvement of one in regulating genes involved in metastasis. 10.1073/pnas.0801130105}, author = {Sengupta, Srikumar and den Boon, Johan A. and Chen, I-How and Newton, Michael A. and Stanhope, Stephen A. and Cheng, Yu-Juen and Chen, Chien-Jen and Hildesheim, Allan and Sugden, Bill and Ahlquist, Paul}, citeulike-article-id = {2630949}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.0801130105}, citeulike-linkout-1 = {http://www.pnas.org/cgi/content/abstract/105/15/5874}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/18390668}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=18390668}, day = {4}, doi = {10.1073/pnas.0801130105}, journal = {Proceedings of the National Academy of Sciences}, keywords = {rnai}, month = {April}, pages = {0801130105+}, posted-at = {2008-04-05 03:25:47}, priority = {2}, title = {MicroRNA 29c is down-regulated in nasopharyngeal carcinomas, up-regulating mRNAs encoding extracellular matrix proteins}, url = {http://dx.doi.org/10.1073/pnas.0801130105}, year = {2008} }

TY - JOUR ID - citeulike:2630949 L3 - citeulike-article-id:2630949 N2 - Using highly sensitive microarray-based procedures, we identified eight microRNAs (miRNAs) showing robust differential expression between 31 laser-capture-microdissected nasopharyngeal carcinomas (NPCs) and 10 normal healthy nasopharyngeal epithelial samples. In particular, miRNA mir-29c was expressed at one-fifth the levels in tumors as in normal epithelium. In NPC tumors, the lower mir-29c levels correlated with higher levels of multiple mRNAs whose 3' UTRs can bind mir-29c at target sequences conserved across many vertebrates. In cultured cells, introduction of mir-29c down-regulated these genes at the level of mRNA and inhibited expression of luciferase encoded by vectors having the 3' UTRs of these genes. Moreover, for each of several genes tested, mutating the mir-29c target sites in the 3' UTR abrogated mir-29c-induced inhibition of luciferase expression. Most of the mir-29c-targeted genes identified encode extracellular matrix proteins, including multiple collagens and laminin {gamma}1, that are associated with tumor cell invasiveness and metastatic potential, prominent characteristics of NPC. Thus, we identify eight miRNAs differentially expressed in NPC and demonstrate the involvement of one in regulating genes involved in metastasis. 10.1073/pnas.0801130105 JF - Proceedings of the National Academy of Sciences TI - MicroRNA 29c is down-regulated in nasopharyngeal carcinomas, up-regulating mRNAs encoding extracellular matrix proteins SP - 0801130105 KW - rnai AU - Sengupta, Srikumar AU - den Boon, Johan AU - Chen, I-How AU - Newton, Michael AU - Stanhope, Stephen AU - Cheng, Yu-Juen AU - Chen, Chien-Jen AU - Hildesheim, Allan AU - Sugden, Bill AU - Ahlquist, Paul PY - 2008/04/04/ UR - http://dx.doi.org/10.1073/pnas.0801130105 ER -