Novel cationic liposome formulation for the delivery of an oligonucleotide decoy to NF-kappaB into activated macrophages. Export

@article{citeulike:2811408, abstract = {Nuclear factor-kappaB (NF-kappaB) is involved in several pathological processes, such as inflammation. Pro-inflammatory genes expression can be down-regulated by using an oligonucleotide (ODN) decoy to NF-kappaB. Cationic liposomes are largely used to improve ODN uptake into cells, although a higher transfection efficiency and a lower toxicity are required to use them in therapy. In this work, we investigated the potential of a novel liposome formulation, based on the recently synthesised cationic lipid (2,3-didodecyloxypropyl) (2-hydroxyethyl) dimethylammonium bromide (DE), as the delivery system for a double stranded ODN decoy to NF-kappaB. Liposomes composed of DE or DE mixed with 1,2-dioleyl-sn-glycero-3-phosphoethanolamine or cholesterol as helper lipids were complexed with ODN at different +/- charge ratios. In vitro uptake and the effect of ODN, naked or complexed with DE-containing liposomes, were evaluated in lipopolysaccharide-stimulated RAW 264.7 macrophages. The use of helper lipids increased liposome physical stability up to 1 year at 4 degrees C. ODN complexed with DE/cholesterol liposomes, at the +/- charge ratio of 8, showed a limited cytotoxicity and the highest inhibition of nitrite production, inducible nitric oxide synthase protein expression and NF-kappaB/DNA binding activity. Confocal microscopy confirmed a high ODN cell uptake obtained with DE/cholesterol liposomes at the highest +/- charge ratio.}, address = {Dipartimento di Chimica Farmaceutica e Tossicologica, Universit\`{a} degli Studi di Napoli Federico II, Naples, Italy.}, author = {De Rosa, Giuseppe and Stefano, Daniela De D. and Laguardia, Valeria and Arpicco, Silvia and Simeon, Vittorio and Carnuccio, Rosa and Fattal, Elias}, citeulike-article-id = {2811408}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.ejpb.2008.03.012}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/18482831}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=18482831}, day = {29}, doi = {10.1016/j.ejpb.2008.03.012}, issn = {0939-6411}, journal = {European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V}, keywords = {macrophage, nf-kappab, nfkb}, month = {March}, posted-at = {2008-05-19 02:29:07}, priority = {2}, title = {Novel cationic liposome formulation for the delivery of an oligonucleotide decoy to NF-kappaB into activated macrophages.}, url = {http://dx.doi.org/10.1016/j.ejpb.2008.03.012}, year = {2008} }

TY - JOUR ID - citeulike:2811408 L3 - citeulike-article-id:2811408 N2 - Nuclear factor-kappaB (NF-kappaB) is involved in several pathological processes, such as inflammation. Pro-inflammatory genes expression can be down-regulated by using an oligonucleotide (ODN) decoy to NF-kappaB. Cationic liposomes are largely used to improve ODN uptake into cells, although a higher transfection efficiency and a lower toxicity are required to use them in therapy. In this work, we investigated the potential of a novel liposome formulation, based on the recently synthesised cationic lipid (2,3-didodecyloxypropyl) (2-hydroxyethyl) dimethylammonium bromide (DE), as the delivery system for a double stranded ODN decoy to NF-kappaB. Liposomes composed of DE or DE mixed with 1,2-dioleyl-sn-glycero-3-phosphoethanolamine or cholesterol as helper lipids were complexed with ODN at different +/- charge ratios. In vitro uptake and the effect of ODN, naked or complexed with DE-containing liposomes, were evaluated in lipopolysaccharide-stimulated RAW 264.7 macrophages. The use of helper lipids increased liposome physical stability up to 1 year at 4 degrees C. ODN complexed with DE/cholesterol liposomes, at the +/- charge ratio of 8, showed a limited cytotoxicity and the highest inhibition of nitrite production, inducible nitric oxide synthase protein expression and NF-kappaB/DNA binding activity. Confocal microscopy confirmed a high ODN cell uptake obtained with DE/cholesterol liposomes at the highest +/- charge ratio. JF - European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V SN - 0939-6411 AD - Dipartimento di Chimica Farmaceutica e Tossicologica, Università degli Studi di Napoli Federico II, Naples, Italy. TI - Novel cationic liposome formulation for the delivery of an oligonucleotide decoy to NF-kappaB into activated macrophages. KW - macrophage KW - nf-kappab KW - nfkb AU - De Rosa, Giuseppe AU - Stefano, Daniela De AU - Laguardia, Valeria AU - Arpicco, Silvia AU - Simeon, Vittorio AU - Carnuccio, Rosa AU - Fattal, Elias PY - 2008/03/29/ UR - http://dx.doi.org/10.1016/j.ejpb.2008.03.012 ER -