Anti-inflammatory effects of flavonoids: genistein, kaempferol, quercetin, and daidzein inhibit STAT-1 and NF-kappaB activations, whereas flavone, isorhamnetin, naringenin, and pelargonidin inhibit only NF-kappaB activation along with their inhibitory effect on iNOS expression and NO production in activated macrophages. Export

@article{citeulike:2831037, abstract = {In inflammation, bacterial products and proinflammatory cytokines induce the formation of large amounts of nitric oxide (NO) by inducible nitric oxide synthase (iNOS), and compounds that inhibit NO production have anti-inflammatory effects. In the present study, we systematically investigated the effects of 36 naturally occurring flavonoids and related compounds on NO production in macrophages exposed to an inflammatory stimulus (lipopolysaccharide, LPS), and evaluated the mechanisms of action of the effective compounds. Flavone, the isoflavones daidzein and genistein, the flavonols isorhamnetin, kaempferol and quercetin, the flavanone naringenin, and the anthocyanin pelargonidin inhibited iNOS protein and mRNA expression and also NO production in a dose-dependent manner. All eight active compounds inhibited the activation of nuclear factor-kappaB (NF-kappaB), which is a significant transcription factor for iNOS. Genistein, kaempferol, quercetin, and daidzein also inhibited the activation of the signal transducer and activator of transcription 1 (STAT-1), another important transcription factor for iNOS. The present study characterises the effects and mechanisms of naturally occurring phenolic compounds on iNOS expression and NO production in activated macrophages. The results partially explain the pharmacological efficacy of flavonoids as anti-inflammatory compounds.}, address = {The Immunopharmacology Research Group, University of Tampere, Medical School and Tampere University Hospital, Research Unit, Tampere 33014, Finland.}, author = {H\"{a}m\"{a}l\"{a}inen, M. and Nieminen, R. and Vuorela, P. and Heinonen, M. and Moilanen, E.}, citeulike-article-id = {2831037}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/18274639}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=18274639}, issn = {1466-1861}, journal = {Mediators of inflammation}, keywords = {inflammation, macrophage, nf-kappab, no, signal}, posted-at = {2008-05-25 18:13:48}, priority = {2}, title = {Anti-inflammatory effects of flavonoids: genistein, kaempferol, quercetin, and daidzein inhibit STAT-1 and NF-kappaB activations, whereas flavone, isorhamnetin, naringenin, and pelargonidin inhibit only NF-kappaB activation along with their inhibitory effect on iNOS expression and NO production in activated macrophages.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/18274639}, volume = {2007}, year = {2007} }

TY - JOUR ID - citeulike:2831037 L3 - citeulike-article-id:2831037 N2 - In inflammation, bacterial products and proinflammatory cytokines induce the formation of large amounts of nitric oxide (NO) by inducible nitric oxide synthase (iNOS), and compounds that inhibit NO production have anti-inflammatory effects. In the present study, we systematically investigated the effects of 36 naturally occurring flavonoids and related compounds on NO production in macrophages exposed to an inflammatory stimulus (lipopolysaccharide, LPS), and evaluated the mechanisms of action of the effective compounds. Flavone, the isoflavones daidzein and genistein, the flavonols isorhamnetin, kaempferol and quercetin, the flavanone naringenin, and the anthocyanin pelargonidin inhibited iNOS protein and mRNA expression and also NO production in a dose-dependent manner. All eight active compounds inhibited the activation of nuclear factor-kappaB (NF-kappaB), which is a significant transcription factor for iNOS. Genistein, kaempferol, quercetin, and daidzein also inhibited the activation of the signal transducer and activator of transcription 1 (STAT-1), another important transcription factor for iNOS. The present study characterises the effects and mechanisms of naturally occurring phenolic compounds on iNOS expression and NO production in activated macrophages. The results partially explain the pharmacological efficacy of flavonoids as anti-inflammatory compounds. JF - Mediators of inflammation SN - 1466-1861 AD - The Immunopharmacology Research Group, University of Tampere, Medical School and Tampere University Hospital, Research Unit, Tampere 33014, Finland. TI - Anti-inflammatory effects of flavonoids: genistein, kaempferol, quercetin, and daidzein inhibit STAT-1 and NF-kappaB activations, whereas flavone, isorhamnetin, naringenin, and pelargonidin inhibit only NF-kappaB activation along with their inhibitory effect on iNOS expression and NO production in activated macrophages. VL - 2007 KW - inflammation KW - macrophage KW - nf-kappab KW - no KW - signal AU - Hämäläinen, M AU - Nieminen, R AU - Vuorela, P AU - Heinonen, M AU - Moilanen, E PY - 2007/// UR - http://view.ncbi.nlm.nih.gov/pubmed/18274639 ER -