Suppressed Production of Pro-inflammatory Cytokines by LPS-Activated Macrophages after Treatment with Toxoplasma gondii Lysate. Export

@article{citeulike:3372626, abstract = {During Toxoplasma gondii infection, macrophages, dendritic cells, and neutrophils are important sources of pro-inflammatory cytokines from the host. To counteract the pro-inflammatory activities, T. gondii is known to have several mechanisms inducing down-regulation of the host immunity. In the present study, we analyzed the production of proand anti-inflammatory cytokines from a human myelomonocytic cell line, THP-1 cells, in response to treatment with T. gondii lysate or lipopolysaccharide (LPS). Treatment of THP-1 cells with LPS induced production of IL-12, TNF-alpha, IL-8, and IL-10. Co-treatment of THP-1 cells with T. gondii lysate inhibited the LPS-induced IL-12, IL-8 and TNF-alpha expression, but increased the level of IL-10 synergistically. IL-12 and IL-10 production was down-regulated by anti-human toll-like receptor (TLR)-2 and TLR4 antibodies. T. gondii lysate triggered nuclear factor (NF)-kappaB-dependent IL-8 expression in HEK293 cells transfected with TLR2. It is suggested that immunosuppression induced by T. gondii lysate treatment might occur via TLR2-mediated NF-kappaB activation.}, author = {Lee, E. J. and Heo, Y. M. and Choi, J. H. and Song, H. O. and Ryu, J. S. and Ahn, M. H.}, citeulike-article-id = {3372626}, citeulike-linkout-0 = {http://dx.doi.org/10.3347/kjp.2008.46.3.145}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/18830053}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=18830053}, doi = {10.3347/kjp.2008.46.3.145}, issn = {0023-4001}, journal = {The Korean journal of parasitology}, keywords = {inflammation, macrophage}, month = {September}, number = {3}, pages = {145--151}, posted-at = {2008-10-04 01:18:42}, priority = {2}, title = {Suppressed Production of Pro-inflammatory Cytokines by LPS-Activated Macrophages after Treatment with Toxoplasma gondii Lysate.}, url = {http://dx.doi.org/10.3347/kjp.2008.46.3.145}, volume = {46}, year = {2008} }

TY - JOUR ID - citeulike:3372626 L3 - citeulike-article-id:3372626 N2 - During Toxoplasma gondii infection, macrophages, dendritic cells, and neutrophils are important sources of pro-inflammatory cytokines from the host. To counteract the pro-inflammatory activities, T. gondii is known to have several mechanisms inducing down-regulation of the host immunity. In the present study, we analyzed the production of proand anti-inflammatory cytokines from a human myelomonocytic cell line, THP-1 cells, in response to treatment with T. gondii lysate or lipopolysaccharide (LPS). Treatment of THP-1 cells with LPS induced production of IL-12, TNF-alpha, IL-8, and IL-10. Co-treatment of THP-1 cells with T. gondii lysate inhibited the LPS-induced IL-12, IL-8 and TNF-alpha expression, but increased the level of IL-10 synergistically. IL-12 and IL-10 production was down-regulated by anti-human toll-like receptor (TLR)-2 and TLR4 antibodies. T. gondii lysate triggered nuclear factor (NF)-kappaB-dependent IL-8 expression in HEK293 cells transfected with TLR2. It is suggested that immunosuppression induced by T. gondii lysate treatment might occur via TLR2-mediated NF-kappaB activation. IS - 3 JF - The Korean journal of parasitology SN - 0023-4001 EP - 151 TI - Suppressed Production of Pro-inflammatory Cytokines by LPS-Activated Macrophages after Treatment with Toxoplasma gondii Lysate. VL - 46 SP - 145 KW - inflammation KW - macrophage AU - Lee, EJ AU - Heo, YM AU - Choi, JH AU - Song, HO AU - Ryu, JS AU - Ahn, MH PY - 2008/09// UR - http://dx.doi.org/10.3347/kjp.2008.46.3.145 ER -