The consequences of chromosomal aneuploidy on gene expression profiles in a cell line model for prostate carcinogenesis. Export

@article{citeulike:3151003, abstract = {Here we report the genetic characterization of immortalized prostate epithelial cells before and after conversion to tumorigenicity using molecular cytogenetics and microarray technology. We were particularly interested to analyze the consequences of acquired chromosomal aneuploidies with respect to modifications of gene expression profiles. Compared with nontumorigenic but immortalized prostate epithelium, prostate tumor cell lines showed high levels of chromosomal rearrangements that led to gains of 1p, 5, 11q, 12p, 16q, and 20q and losses of 1pter, 11p, 17, 20p, 21, 22, and Y. Of 5700 unique targets on a 6.5K cDNA microarray, approximately 3\% were subject to modification in expression levels; these included GRO-1, -2, IAP-1,- 2, MMP-9, and cyclin D1, which showed increased expression, and TRAIL, BRCA1, and CTNNA, which showed decreased expression. Thirty \% of expression changes occurred in regions the genomic copy number of which remained balanced. Of the remainder, 42\% of down-regulated and 51\% of up-regulated genes mapped to regions present in decreased or increased genomic copy numbers, respectively. A relative gain or loss of a chromosome or chromosomal arm usually resulted in a statistically significant increase or decrease, respectively, in the average expression level of all of the genes on the chromosome. However, of these genes, very few (e.g., 5 of 101 genes on chromosome 11q), and in some instances only two genes (MMP-9 and PROCR on chromosome 20q), were overexpressed by > or =1.7-fold when scored individually. Cluster analysis by gene function suggests that prostate tumorigenesis in these cell line models involves alterations in gene expression that may favor invasion, prevent apoptosis, and promote growth.}, address = {Urologic Oncology Branch, Center for Cancer Research, National Cancer Institute, NIH, 10 Center Drive, Bethesda, MD 20817, USA. phillipj@mail.nih.gov}, author = {Phillips, J. L. and Hayward, S. W. and Wang, Y. and Vasselli, J. and Pavlovich, C. and Padilla-Nash, H. and Pezullo, J. R. and Ghadimi, B. M. and Grossfeld, G. D. and Rivera, A. and Linehan, W. M. and Cunha, G. R. and Ried, T.}, citeulike-article-id = {3151003}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/11719443}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=11719443}, day = {15}, issn = {0008-5472}, journal = {Cancer research}, keywords = {20q, cell\_line, prostate}, month = {November}, number = {22}, pages = {8143--8149}, posted-at = {2008-08-23 14:08:53}, priority = {2}, title = {The consequences of chromosomal aneuploidy on gene expression profiles in a cell line model for prostate carcinogenesis.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/11719443}, volume = {61}, year = {2001} }

TY - JOUR ID - citeulike:3151003 L3 - citeulike-article-id:3151003 N2 - Here we report the genetic characterization of immortalized prostate epithelial cells before and after conversion to tumorigenicity using molecular cytogenetics and microarray technology. We were particularly interested to analyze the consequences of acquired chromosomal aneuploidies with respect to modifications of gene expression profiles. Compared with nontumorigenic but immortalized prostate epithelium, prostate tumor cell lines showed high levels of chromosomal rearrangements that led to gains of 1p, 5, 11q, 12p, 16q, and 20q and losses of 1pter, 11p, 17, 20p, 21, 22, and Y. Of 5700 unique targets on a 6.5K cDNA microarray, approximately 3% were subject to modification in expression levels; these included GRO-1, -2, IAP-1,- 2, MMP-9, and cyclin D1, which showed increased expression, and TRAIL, BRCA1, and CTNNA, which showed decreased expression. Thirty % of expression changes occurred in regions the genomic copy number of which remained balanced. Of the remainder, 42% of down-regulated and 51% of up-regulated genes mapped to regions present in decreased or increased genomic copy numbers, respectively. A relative gain or loss of a chromosome or chromosomal arm usually resulted in a statistically significant increase or decrease, respectively, in the average expression level of all of the genes on the chromosome. However, of these genes, very few (e.g., 5 of 101 genes on chromosome 11q), and in some instances only two genes (MMP-9 and PROCR on chromosome 20q), were overexpressed by > or =1.7-fold when scored individually. Cluster analysis by gene function suggests that prostate tumorigenesis in these cell line models involves alterations in gene expression that may favor invasion, prevent apoptosis, and promote growth. IS - 22 JF - Cancer research SN - 0008-5472 AD - Urologic Oncology Branch, Center for Cancer Research, National Cancer Institute, NIH, 10 Center Drive, Bethesda, MD 20817, USA. phillipj@mail.nih.gov EP - 8149 TI - The consequences of chromosomal aneuploidy on gene expression profiles in a cell line model for prostate carcinogenesis. VL - 61 SP - 8143 KW - 20q KW - cell_line KW - prostate AU - Phillips, JL AU - Hayward, SW AU - Wang, Y AU - Vasselli, J AU - Pavlovich, C AU - Padilla-Nash, H AU - Pezullo, JR AU - Ghadimi, BM AU - Grossfeld, GD AU - Rivera, A AU - Linehan, WM AU - Cunha, GR AU - Ried, T PY - 2001/11/15/ UR - http://view.ncbi.nlm.nih.gov/pubmed/11719443 ER -