Analysis of the distribution of MRI contrast agents in the livers of small animals by means of complementary microscopies Export

@article{citeulike:3244040, abstract = {Magnetic resonance imaging (MRI) contrast agents contain magnetic molecules such as iron (Fe) or gadolinium (Gd) that are injected in vivo into rats or mice to study their distribution inside the liver. Fluorescent europium (Eu) can be used as a model of Gd to obtain comparable information of this distribution of corresponding contrast agents. In a similar approach, Fe can be attached to Texas Red and used as a model of ferumoxides and be detected by fluorescence.To combine and compare the advantages of different microscopic imaging modes, characterization studies were carried out by means of a confocal laser scanning microscope (CLSM), a secondary ion mass spectrometric (SIMS) microscope, and an electron energy loss spectrometric (EELS) microscope. In the case of CLSM, the locations of fluorescent signals inside preparations were determined by factor analysis of biomedical image sequences (FAMIS) and selection of image sequences at emission.By CLSM and FAMIS, we distinguished chelated Eu and Texas Red attached to Fe. By SIMS microscopy, we distinguished Eu and Gd of chlorides and chelates and Fe of a ferumoxide. By EELS microscopy, we distinguished Eu and Gd of chlorides.Analysis of compounds inside correlative specimens by means of CLSM, SIMS, and EELS microscopes provided complementary results. Cytometry Part A 51A:97-106, 2003. {\copyright} 2003 Wiley-Liss, Inc.}, address = {INSERM U494, CHU Piti\'{e}-Salp\'{e}tri\`{e}re, 75634 Paris Cedex 13, France; Institut de Protection et de Suret\'{e} Nucl\'{e}aire, 92265 Fontenay-aux-Roses Cedex, France; INSERM U498, H\^{o}pital du Bocage, Dijon, France; Laboratoire de Biophysique des Traceurs, CHU Bichat, Paris, France; Centre Commun de Quantim\'{e}trie, Universit\'{e} Lyon 1, Lyon, France; Laboratoire de Microscopie Mol\'{e}culaire et Cellulaire, CNRS UMR 8532, Institut Gustave-Roussy, Villejuif, France; Service de M\'{e}decine Nucl\'{e}aire, CHU Kremlin Bic\`{e}tre, France}, author = {Kahn, E. and Tessier, C. and Lizard, G. and Petiet, A. and Bernengo, J. C. and Coulaud, D. and Fourr\'{e}, C. and Frouin, F. and Cl\'{e}ment, O. and Jourdain, J. R. and Delain, E. and Guiraud-Vitaux, F. and Colas-Linhart, N. and Siauve, N. and Cuenod, C. A. and Frija, G. and Todd-Pokropek, A.}, citeulike-article-id = {3244040}, citeulike-linkout-0 = {http://dx.doi.org/10.1002/cyto.a.10017}, citeulike-linkout-1 = {http://www3.interscience.wiley.com/cgi-bin/abstract/102526736/ABSTRACT}, doi = {10.1002/cyto.a.10017}, journal = {Cytometry}, keywords = {agents, contrast, liver, microscopy, mri}, number = {2}, pages = {97--106}, posted-at = {2008-09-12 08:27:17}, priority = {5}, title = {Analysis of the distribution of MRI contrast agents in the livers of small animals by means of complementary microscopies}, url = {http://dx.doi.org/10.1002/cyto.a.10017}, volume = {51A}, year = {2003} }

TY - JOUR ID - citeulike:3244040 L3 - citeulike-article-id:3244040 N2 - Magnetic resonance imaging (MRI) contrast agents contain magnetic molecules such as iron (Fe) or gadolinium (Gd) that are injected in vivo into rats or mice to study their distribution inside the liver. Fluorescent europium (Eu) can be used as a model of Gd to obtain comparable information of this distribution of corresponding contrast agents. In a similar approach, Fe can be attached to Texas Red and used as a model of ferumoxides and be detected by fluorescence.To combine and compare the advantages of different microscopic imaging modes, characterization studies were carried out by means of a confocal laser scanning microscope (CLSM), a secondary ion mass spectrometric (SIMS) microscope, and an electron energy loss spectrometric (EELS) microscope. In the case of CLSM, the locations of fluorescent signals inside preparations were determined by factor analysis of biomedical image sequences (FAMIS) and selection of image sequences at emission.By CLSM and FAMIS, we distinguished chelated Eu and Texas Red attached to Fe. By SIMS microscopy, we distinguished Eu and Gd of chlorides and chelates and Fe of a ferumoxide. By EELS microscopy, we distinguished Eu and Gd of chlorides.Analysis of compounds inside correlative specimens by means of CLSM, SIMS, and EELS microscopes provided complementary results. Cytometry Part A 51A:97-106, 2003. © 2003 Wiley-Liss, Inc. IS - 2 JF - Cytometry AD - INSERM U494, CHU Pitié-Salpétrière, 75634 Paris Cedex 13, France; Institut de Protection et de Sureté Nucléaire, 92265 Fontenay-aux-Roses Cedex, France; INSERM U498, Hôpital du Bocage, Dijon, France; Laboratoire de Biophysique des Traceurs, CHU Bichat, Paris, France; Centre Commun de Quantimétrie, Université Lyon 1, Lyon, France; Laboratoire de Microscopie Moléculaire et Cellulaire, CNRS UMR 8532, Institut Gustave-Roussy, Villejuif, France; Service de Médecine Nucléaire, CHU Kremlin Bicètre, France EP - 106 TI - Analysis of the distribution of MRI contrast agents in the livers of small animals by means of complementary microscopies VL - 51A SP - 97 KW - agents KW - contrast KW - liver KW - microscopy KW - mri AU - Kahn, E AU - Tessier, C AU - Lizard, G AU - Petiet, A AU - Bernengo, JC AU - Coulaud, D AU - Fourré, C AU - Frouin, F AU - Clément, O AU - Jourdain, JR AU - Delain, E AU - Guiraud-Vitaux, F AU - Colas-Linhart, N AU - Siauve, N AU - Cuenod, CA AU - Frija, G AU - Todd-Pokropek, A PY - 2003/// UR - http://dx.doi.org/10.1002/cyto.a.10017 ER -