Molecular epidemiology of rabies virus strains isolated from wild canids in Northeastern Brazil Export

@article{citeulike:3358932, abstract = {Rabies in wild canids in Northeastern Brazil is frequent and has been reported for some time, with episodes of rabies transmission from these animals to humans also reported. In this study, we analyzed the antigenic and genetic profiles of the rabies virus nucleoprotein gene, isolated from 20 samples taken from domestic animals and wild canids located in the Northeastern region of Brazil. All viruses isolated from domestic animals (dogs and cats) belonged to the antigenic variant 2 (AgV2). Among the wild animal samples, only four were AgV2, and nine showed a divergent antigenic profile. Phylogenetic analysis revealed two Brazilian clusters. Cluster 1 (Brazilian domestic carnivore-related strains) showed two subclusters, called 1A and 1B, and cluster 2 (Brazilian wild canid-related strains) also showed two subclusters, called 2A and 2B. The majority of the samples with divergent antigenic strains segregated into subcluster 2A. The intracluster identity of cluster 1 was 95.6\% and that of cluster 2, 92.4\%. When clusters 1 and 2 were compared, an identity of 88.6\% was found. The genetic analysis of wild canid samples performed in this study indicates that there are two distinct rabies cycles among canids in Brazil, one represented by domestic canids and the other by wild canids. This study shows that the virus samples isolated in Northeastern Brazil are region and species-specific.}, author = {Carnieli, Pedro and Brand\~{a}oa, Paulo E. and Carrieria, Maria L. and Castilhoa, Juliana G. and Macedoa, Carla I. and Machadob, Lindenberg M. and Rangelc, Norm\'{e}lia and de Carvalhod, Rosangela C. and de Carvalhod, Vania A. and Montebelloe, Lucia and Wadae, Marcelo and Kotaita, Ivanete}, citeulike-article-id = {3358932}, citeulike-linkout-0 = {http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T32-4JYKMK6-1&_user=10&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_version=1&_urlVersion=0&_userid=10&md5=11507e5adb3c5a8724d3a1c002716ee0}, journal = {Virus Research}, keywords = {antigenic, brazil, canids, gene, n, phylogeny, rabies, typing, wild}, month = {September}, number = {1-2}, pages = {113--120}, posted-at = {2008-09-30 15:09:40}, priority = {2}, title = {Molecular epidemiology of rabies virus strains isolated from wild canids in Northeastern Brazil}, url = {http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T32-4JYKMK6-1&_user=10&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_version=1&_urlVersion=0&_userid=10&md5=11507e5adb3c5a8724d3a1c002716ee0}, volume = {120}, year = {2006} }

TY - JOUR ID - citeulike:3358932 L3 - citeulike-article-id:3358932 N2 - Rabies in wild canids in Northeastern Brazil is frequent and has been reported for some time, with episodes of rabies transmission from these animals to humans also reported. In this study, we analyzed the antigenic and genetic profiles of the rabies virus nucleoprotein gene, isolated from 20 samples taken from domestic animals and wild canids located in the Northeastern region of Brazil. All viruses isolated from domestic animals (dogs and cats) belonged to the antigenic variant 2 (AgV2). Among the wild animal samples, only four were AgV2, and nine showed a divergent antigenic profile. Phylogenetic analysis revealed two Brazilian clusters. Cluster 1 (Brazilian domestic carnivore-related strains) showed two subclusters, called 1A and 1B, and cluster 2 (Brazilian wild canid-related strains) also showed two subclusters, called 2A and 2B. The majority of the samples with divergent antigenic strains segregated into subcluster 2A. The intracluster identity of cluster 1 was 95.6% and that of cluster 2, 92.4%. When clusters 1 and 2 were compared, an identity of 88.6% was found. The genetic analysis of wild canid samples performed in this study indicates that there are two distinct rabies cycles among canids in Brazil, one represented by domestic canids and the other by wild canids. This study shows that the virus samples isolated in Northeastern Brazil are region and species-specific. IS - 1-2 JF - Virus Research EP - 120 TI - Molecular epidemiology of rabies virus strains isolated from wild canids in Northeastern Brazil VL - 120 SP - 113 KW - antigenic KW - brazil KW - canids KW - gene KW - n KW - phylogeny KW - rabies KW - typing KW - wild AU - Carnieli, Pedro AU - Brandãoa, Paulo AU - Carrieria, Maria AU - Castilhoa, Juliana AU - Macedoa, Carla AU - Machadob, Lindenberg AU - Rangelc, Normélia AU - de Carvalhod, Rosangela AU - de Carvalhod, Vania AU - Montebelloe, Lucia AU - Wadae, Marcelo AU - Kotaita, Ivanete PY - 2006/09// UR - http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T32-4JYKMK6-1&_user=10&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_version=1&_urlVersion=0&_userid=10&md5=11507e5adb3c5a8724d3a1c002716ee0 ER -