Prenatal exposure to bisphenol A up-regulates immune responses, including T helper 1 and T helper 2 responses, in mice Export

@article{citeulike:3421524, abstract = {The effect of prenatal exposure to bisphenol A (BPA) on the immune system in mice was investigated. Virgin female mice were fed varying doses of BPA, on a daily basis, over a period of 18 days commencing on the day of pairing with stud males (day 0). On day 77, their male offspring of 8 weeks were immunized with hen egg lysozyme (HEL). Three weeks later, anti-HEL immunoglobulin G (IgG) in sera, and proliferative responses of spleen cells to the antigen, were measured. Anti-HEL IgG2a and interferon-03B3 (IFN-03B3), secreted from splenic lymphocytes, were measured as indicators of T helper 1 (Th1) immune responses, while anti-HEL IgG1 and interleukin-4 (IL-4) were measured as indicators of Th2 responses. The results showed that fetal exposure to BPA was followed by significant increases in anti-HEL IgG as well as antigen-specific cell proliferation. Both Th1 responses (including anti-HEL IgG2a and IFN-03B3 production) and Th2 responses (including anti-HEL IgG1 and IL-4 production) were augmented by prenatal exposure to BPA, although the augmentation of Th1 responses appeared to be greater than that of Th2 responses. Two-colour flow cytometric analysis showed that mice exposed prenatally to BPA had 29\% and 100\% more splenic CD3+ CD4+ and CD3+ CD8+ cells, respectively, than control animals. Similar results were obtained from females whose mothers had consumed BPA during pregnancy. These results suggest that prenatal exposure to BPA may result in the up-regulation of immune responses, especially Th1 responses, in adulthood.}, address = {Department of Pharmacology, Kobe Pharmaceutical University, Kobe, Japan; Microbiology; Immunology, Saga Medical School, Saga, Japan; Pathophysiology Research Team, National Institute for Environmental Studies, Tsukuba, Japan; Clinical Toxicology and Metabolism; Immunology and Microbiology, Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Tobetsu, Hokkaido, Japan}, author = {Yoshino, Shin and Yamaki, Kouya and Li, Xiaojuan and Sai, Tao and Yanagisawa, Rie and Takano, Hirohisa and Taneda, Shinji and Hayashi, Hideyuki and Mori, Yoki}, citeulike-article-id = {3421524}, citeulike-linkout-0 = {http://dx.doi.org/10.1111/j.1365-2567.2004.01900.x}, citeulike-linkout-1 = {http://www3.interscience.wiley.com/cgi-bin/abstract/118753627/ABSTRACT}, doi = {10.1111/j.1365-2567.2004.01900.x}, journal = {Immunology}, keywords = {estrogen, immunology, mice, prenatal, response, th1, th2, xenoestrogen, xenoestrogen-immune}, number = {3}, pages = {489--495}, posted-at = {2008-10-16 18:57:00}, priority = {2}, title = {Prenatal exposure to bisphenol A up-regulates immune responses, including T helper 1 and T helper 2 responses, in mice}, url = {http://dx.doi.org/10.1111/j.1365-2567.2004.01900.x}, volume = {112}, year = {2004} }

TY - JOUR ID - citeulike:3421524 L3 - citeulike-article-id:3421524 N2 - The effect of prenatal exposure to bisphenol A (BPA) on the immune system in mice was investigated. Virgin female mice were fed varying doses of BPA, on a daily basis, over a period of 18 days commencing on the day of pairing with stud males (day 0). On day 77, their male offspring of 8 weeks were immunized with hen egg lysozyme (HEL). Three weeks later, anti-HEL immunoglobulin G (IgG) in sera, and proliferative responses of spleen cells to the antigen, were measured. Anti-HEL IgG2a and interferon-03B3 (IFN-03B3), secreted from splenic lymphocytes, were measured as indicators of T helper 1 (Th1) immune responses, while anti-HEL IgG1 and interleukin-4 (IL-4) were measured as indicators of Th2 responses. The results showed that fetal exposure to BPA was followed by significant increases in anti-HEL IgG as well as antigen-specific cell proliferation. Both Th1 responses (including anti-HEL IgG2a and IFN-03B3 production) and Th2 responses (including anti-HEL IgG1 and IL-4 production) were augmented by prenatal exposure to BPA, although the augmentation of Th1 responses appeared to be greater than that of Th2 responses. Two-colour flow cytometric analysis showed that mice exposed prenatally to BPA had 29% and 100% more splenic CD3+ CD4+ and CD3+ CD8+ cells, respectively, than control animals. Similar results were obtained from females whose mothers had consumed BPA during pregnancy. These results suggest that prenatal exposure to BPA may result in the up-regulation of immune responses, especially Th1 responses, in adulthood. IS - 3 JF - Immunology AD - Department of Pharmacology, Kobe Pharmaceutical University, Kobe, Japan; Microbiology; Immunology, Saga Medical School, Saga, Japan; Pathophysiology Research Team, National Institute for Environmental Studies, Tsukuba, Japan; Clinical Toxicology and Metabolism; Immunology and Microbiology, Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Tobetsu, Hokkaido, Japan EP - 495 TI - Prenatal exposure to bisphenol A up-regulates immune responses, including T helper 1 and T helper 2 responses, in mice VL - 112 SP - 489 KW - estrogen KW - immunology KW - mice KW - prenatal KW - response KW - th1 KW - th2 KW - xenoestrogen KW - xenoestrogen-immune AU - Yoshino, Shin AU - Yamaki, Kouya AU - Li, Xiaojuan AU - Sai, Tao AU - Yanagisawa, Rie AU - Takano, Hirohisa AU - Taneda, Shinji AU - Hayashi, Hideyuki AU - Mori, Yoki PY - 2004/// UR - http://dx.doi.org/10.1111/j.1365-2567.2004.01900.x ER -