Inhibition of caspase-8 activity reduces IFN-gamma expression by T cells from Leishmania major infection. Export

@article{citeulike:3477113, abstract = {Following infection with Leishmania major, T cell activation and apoptosis can be detected in draining lymph nodes of C57BL/6-infected mice. We investigated the mechanisms involved in apoptosis and cytokine expression following T cell activation. After two weeks of infection, apoptotic T cells were not detected in draining lymph nodes but activation with anti-CD3 induced apoptosis in both CD4 and CD8 T cells. Treatment with anti-Fas Ligand, caspase-8 or caspase- 9 inhibitors did not block activation-induced T-cell death. We also investigated whether the blockade of caspase-8 activity would affect the expression of type-1 or type-2 cytokines. At early stages of infection, both CD4 and CD8 T cells expressed IFN-gamma upon activation. Treatment with the caspase-8 inhibitor zIETD-fmk (benzyl-oxycarbonyl-Ile- Glu(OMe)-Thr-Asp(OMe)-fluoromethyl ketone) reduced the proportion of CD8 T cells and IFN-gamma expression in both CD4 and CD8 T cells. We conclude that a non apoptotic role of caspase-8 activity may be required for T cell-mediated type-1 responses during L. major infection.}, author = {Pereira, W. F. and Guillermo, L. V. and Ribeiro-Gomes, F. L. and Lopes, M. F.}, citeulike-article-id = {3477113}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/18345381}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=18345381}, issn = {0001-3765}, journal = {Anais da Academia Brasileira de Ci\^{e}ncias}, keywords = {breast, cancer, caspase-8, endemic, evolepi, ifn-gamma, leishmania, t-cell, thelist}, month = {March}, number = {1}, pages = {129--136}, posted-at = {2008-11-03 22:32:46}, priority = {2}, title = {Inhibition of caspase-8 activity reduces IFN-gamma expression by T cells from Leishmania major infection.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/18345381}, volume = {80}, year = {2008} }

TY - JOUR ID - citeulike:3477113 L3 - citeulike-article-id:3477113 N2 - Following infection with Leishmania major, T cell activation and apoptosis can be detected in draining lymph nodes of C57BL/6-infected mice. We investigated the mechanisms involved in apoptosis and cytokine expression following T cell activation. After two weeks of infection, apoptotic T cells were not detected in draining lymph nodes but activation with anti-CD3 induced apoptosis in both CD4 and CD8 T cells. Treatment with anti-Fas Ligand, caspase-8 or caspase- 9 inhibitors did not block activation-induced T-cell death. We also investigated whether the blockade of caspase-8 activity would affect the expression of type-1 or type-2 cytokines. At early stages of infection, both CD4 and CD8 T cells expressed IFN-gamma upon activation. Treatment with the caspase-8 inhibitor zIETD-fmk (benzyl-oxycarbonyl-Ile- Glu(OMe)-Thr-Asp(OMe)-fluoromethyl ketone) reduced the proportion of CD8 T cells and IFN-gamma expression in both CD4 and CD8 T cells. We conclude that a non apoptotic role of caspase-8 activity may be required for T cell-mediated type-1 responses during L. major infection. IS - 1 JF - Anais da Academia Brasileira de Ciências SN - 0001-3765 EP - 136 TI - Inhibition of caspase-8 activity reduces IFN-gamma expression by T cells from Leishmania major infection. VL - 80 SP - 129 KW - breast KW - cancer KW - caspase-8 KW - endemic KW - evolepi KW - ifn-gamma KW - leishmania KW - t-cell KW - thelist AU - Pereira, WF AU - Guillermo, LV AU - Ribeiro-Gomes, FL AU - Lopes, MF PY - 2008/03// UR - http://view.ncbi.nlm.nih.gov/pubmed/18345381 ER -