High levels of oxidative DNA damage in lymphocyte DNA of premenopausal breast cancer patients from Egypt Export

@article{citeulike:3658761, abstract = {Egypt shows a parallel increase in premenopausal breast cancer and environmental pollution. The purpose of this study is to explore a possible relationship between oxidative DNA damage, urinary estrogen metabolites and breast cancer in Egyptian premenopausal women. We conducted a pilot study of Egyptian breast cancer involving 29 cases and 32 controls and analysed lymphocyte DNA levels of 7,8-dihydro-8-oxo-2\&prime;-deoxyguanine (8-oxo-dG), a measure of oxidative DNA damage using high performance liquid chromatography with electro-chemical detection (HPLC-ECD) method. We analysed levels of urinary estrogen metabolites, 2-hydroxyestrone (2-OHE) and 16\&agr;-hydroxyestrone (16\&agr;-OHE) by an enzyme immuno assay. We also collected residential, occupational, and reproductive histories of all study subjects. We detected, in all subjects, exceptionally high levels of 8-oxo-dG and thus oxidative DNA damage, the levels (mean 8-oxo-dG/10⁵ dG\&hairsp;±\&hairsp;SD) were significantly (<i>P</i>\&hairsp;\&lt;\&hairsp;0.01) higher in breast cancer cases (139.4\&hairsp;±\&hairsp;78.4) than in controls (60.9\&hairsp;±\&hairsp;51.5). Urinary 2-OHE and 16\&agr;-OHE or their ratio was not significantly different between cases and controls. However, 8-oxo-dG levels were positively correlated (<i>P</i>\&hairsp;\&lt;\&hairsp;0.05) with 2-OHE and 16\&agr;-OHE from cases while controls showed a negative correlation (<i>P</i>\&hairsp;\&lt;\&hairsp;0.05). Urban residence (Odds Ratio [OR] 3.1; Confidence interval [CI], 1.1\&hairsp;–\&hairsp;9.3), infertility (OR [9.8]; CI [1.1\&hairsp;–\&hairsp;89.7]), age (OR [2.6]; CI [1.4\&hairsp;–\&hairsp;4.6]) and 8-oxo-dG (OR 5.8; CI 1.9\&hairsp;–\&hairsp;17.5) levels were found to be significant predictors of breast cancer. Our finding of exceptionally high levels of 8-oxo-dG, a common result of oxidative DNA damage, warrant future studies on a larger population of premenopausal women in Egypt with consideration of other susceptibility markers and dietary characteristics.}, author = {Soliman, Amr S. and Vulimiri, Suryanarayana V. and Kleiner, Heather E. and Shen, Jianjun and Eissa, Saad and Morad, Magda and Taha, Hala and Lukmanji, Farzana and Li, Donghui and Johnston, Dennis A. and Lo, Herng-Hsang and Lau, Serrine and Digiovanni, John and Bondy, Melissa L.}, citeulike-article-id = {3658761}, citeulike-linkout-0 = {http://dx.doi.org/10.1080/0960312042000209534}, comment = {The authors state that all the study subjects had a high level of estrogen metabolites. Was this because the subjects also had a higher lever of estrogen than the Caucasians (who were probably the source of the normal distribution for the estrogen metabolites)? Worth thinking about...}, doi = {10.1080/0960312042000209534}, journal = {International Journal of Environmental Health Research}, keywords = {africa, breast, cancer, egypt, estrogen, metabolite, premenopausal, thelist}, number = {2}, pages = {121--134}, posted-at = {2008-11-22 03:04:25}, priority = {2}, publisher = {Taylor \& Francis}, title = {High levels of oxidative DNA damage in lymphocyte DNA of premenopausal breast cancer patients from Egypt}, url = {http://dx.doi.org/10.1080/0960312042000209534}, volume = {14}, year = {2004} }

TY - JOUR ID - citeulike:3658761 L3 - citeulike-article-id:3658761 N2 - Egypt shows a parallel increase in premenopausal breast cancer and environmental pollution. The purpose of this study is to explore a possible relationship between oxidative DNA damage, urinary estrogen metabolites and breast cancer in Egyptian premenopausal women. We conducted a pilot study of Egyptian breast cancer involving 29 cases and 32 controls and analysed lymphocyte DNA levels of 7,8-dihydro-8-oxo-2&prime;-deoxyguanine (8-oxo-dG), a measure of oxidative DNA damage using high performance liquid chromatography with electro-chemical detection (HPLC-ECD) method. We analysed levels of urinary estrogen metabolites, 2-hydroxyestrone (2-OHE) and 16&agr;-hydroxyestrone (16&agr;-OHE) by an enzyme immuno assay. We also collected residential, occupational, and reproductive histories of all study subjects. We detected, in all subjects, exceptionally high levels of 8-oxo-dG and thus oxidative DNA damage, the levels (mean 8-oxo-dG/10⁵ dG&hairsp;±&hairsp;SD) were significantly (<i>P</i>&hairsp;&lt;&hairsp;0.01) higher in breast cancer cases (139.4&hairsp;±&hairsp;78.4) than in controls (60.9&hairsp;±&hairsp;51.5). Urinary 2-OHE and 16&agr;-OHE or their ratio was not significantly different between cases and controls. However, 8-oxo-dG levels were positively correlated (<i>P</i>&hairsp;&lt;&hairsp;0.05) with 2-OHE and 16&agr;-OHE from cases while controls showed a negative correlation (<i>P</i>&hairsp;&lt;&hairsp;0.05). Urban residence (Odds Ratio [OR] 3.1; Confidence interval [CI], 1.1&hairsp;–&hairsp;9.3), infertility (OR [9.8]; CI [1.1&hairsp;–&hairsp;89.7]), age (OR [2.6]; CI [1.4&hairsp;–&hairsp;4.6]) and 8-oxo-dG (OR 5.8; CI 1.9&hairsp;–&hairsp;17.5) levels were found to be significant predictors of breast cancer. Our finding of exceptionally high levels of 8-oxo-dG, a common result of oxidative DNA damage, warrant future studies on a larger population of premenopausal women in Egypt with consideration of other susceptibility markers and dietary characteristics. IS - 2 JF - International Journal of Environmental Health Research EP - 134 TI - High levels of oxidative DNA damage in lymphocyte DNA of premenopausal breast cancer patients from Egypt PB - Taylor & Francis VL - 14 SP - 121 KW - africa KW - breast KW - cancer KW - egypt KW - estrogen KW - metabolite KW - premenopausal KW - thelist N1 - The authors state that all the study subjects had a high level of estrogen metabolites. Was this because the subjects also had a higher lever of estrogen than the Caucasians (who were probably the source of the normal distribution for the estrogen metabolites)? Worth thinking about... AU - Soliman, Amr AU - Vulimiri, Suryanarayana AU - Kleiner, Heather AU - Shen, Jianjun AU - Eissa, Saad AU - Morad, Magda AU - Taha, Hala AU - Lukmanji, Farzana AU - Li, Donghui AU - Johnston, Dennis AU - Lo, Herng-Hsang AU - Lau, Serrine AU - Digiovanni, John AU - Bondy, Melissa PY - 2004/// UR - http://dx.doi.org/10.1080/0960312042000209534 ER -