Bisphenol A Exposure Induces Apoptosis and Up-regulation of Fas/FasL and Caspase-3 Expression in the Testes of Mice. Export

@article{citeulike:4052839, abstract = {There are controversies about adverse effects of bisphenol A (BPA), a ubiquitous xenoestrogen, on reproduction and development of male animals. To understand BPA action and assess its risk more completely, we examined the impact of BPA at high doses on the testes of pubertal male Kunming (China) mice. BPA at 0 (control), 160, 480 and 960 mg/kg/day was given by gavage to mice from postnatal days (PND) 31 to 44, followed by observation of morphology and detection of apoptosis and expressions of Fas/FasL and active caspase-3 on PND 45, 60 and 90 by TUNEL, immunohistochemistry and Western blotting. There was no effect of BPA at 160 mg/kg/day, however, at 480 and 960 mg/kg/day there was underdevelopment of testes and disruption of spermatogenesis. There were many apoptotic Leydig and germ cells in the testes with apoptotic indices being significantly increased compared with controls. The expression of Fas and active caspase-3 was localized in the same cell types as apoptosis occurred, and expression levels of Fas, FasL and active caspase-3 were significantly increased compared with controls. The disturbed spermatogenesis, apoptosis and up-regulation of Fas, FasL and active caspase-3 expression persisted to PND 90. The results suggest that high-dose BPA induces apoptosis of Leydig and germ cells in the mouse testis through the Fas signaling pathway. Therefore, there is concern about reproductive health for humans occupationally exposed to high levels of BPA.}, author = {Li, Yuan-Jie J. and Song, Tian-Bao B. and Cai, Yan-Yan Y. and Zhou, Jin-Song S. and Song, Xin and Zhao, Xuan and Wu, Xiao-Lin L.}, citeulike-article-id = {4052839}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/19193734}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=19193734}, day = {4}, issn = {1096-0929}, journal = {Toxicological sciences : an official journal of the Society of Toxicology}, keywords = {apoptosis, bisphenol-a, caspase-3, fas, fasl, mice, testes, xenoestrogen}, month = {February}, posted-at = {2009-02-15 02:30:34}, priority = {2}, title = {Bisphenol A Exposure Induces Apoptosis and Up-regulation of Fas/FasL and Caspase-3 Expression in the Testes of Mice.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/19193734}, year = {2009} }

TY - JOUR ID - citeulike:4052839 L3 - citeulike-article-id:4052839 N2 - There are controversies about adverse effects of bisphenol A (BPA), a ubiquitous xenoestrogen, on reproduction and development of male animals. To understand BPA action and assess its risk more completely, we examined the impact of BPA at high doses on the testes of pubertal male Kunming (China) mice. BPA at 0 (control), 160, 480 and 960 mg/kg/day was given by gavage to mice from postnatal days (PND) 31 to 44, followed by observation of morphology and detection of apoptosis and expressions of Fas/FasL and active caspase-3 on PND 45, 60 and 90 by TUNEL, immunohistochemistry and Western blotting. There was no effect of BPA at 160 mg/kg/day, however, at 480 and 960 mg/kg/day there was underdevelopment of testes and disruption of spermatogenesis. There were many apoptotic Leydig and germ cells in the testes with apoptotic indices being significantly increased compared with controls. The expression of Fas and active caspase-3 was localized in the same cell types as apoptosis occurred, and expression levels of Fas, FasL and active caspase-3 were significantly increased compared with controls. The disturbed spermatogenesis, apoptosis and up-regulation of Fas, FasL and active caspase-3 expression persisted to PND 90. The results suggest that high-dose BPA induces apoptosis of Leydig and germ cells in the mouse testis through the Fas signaling pathway. Therefore, there is concern about reproductive health for humans occupationally exposed to high levels of BPA. JF - Toxicological sciences : an official journal of the Society of Toxicology SN - 1096-0929 TI - Bisphenol A Exposure Induces Apoptosis and Up-regulation of Fas/FasL and Caspase-3 Expression in the Testes of Mice. KW - apoptosis KW - bisphenol-a KW - caspase-3 KW - fas KW - fasl KW - mice KW - testes KW - xenoestrogen AU - Li, Yuan-Jie AU - Song, Tian-Bao AU - Cai, Yan-Yan AU - Zhou, Jin-Song AU - Song, Xin AU - Zhao, Xuan AU - Wu, Xiao-Lin PY - 2009/02/04/ UR - http://view.ncbi.nlm.nih.gov/pubmed/19193734 ER -