Inhibition of Human Polymorphonuclear Cell Oxidative Burst by 17-&#x03B2;-estradiol and 2,3,7,8-tetrachlorodibenzo-<i>p</i>-dioxin Export

@article{citeulike:4085513, abstract = {Abrahams VM, Collins JE, Wira CR, Fanger MW, Yeaman GR. Inhibition of human polymorphonuclear cell oxidative burst by 17-03B2-estradiol and 2,3,7,8-tetrachlorodibenzo-p-dioxin. AJRI 2003; 50:463ndash;472 {\copyright} Blackwell Munksgaard, 2003Problem:  Polymorphonuclear cell (PMN) function may be directly influenced by 17-03B2-estradiol and the endocrine disruptor, 2,3,7,8-tetrachloro-dibenzo-p-dioxin (TCDD). This may have significant consequences on PMN function within the female reproductive tract. This study evaluated the effects of 17-03B2-estradiol and TCDD on PMN oxidative burst.Method of study:  Peripheral blood PMN were isolated from normal male donors. Following treatment with 17-03B2-estradiol, TCDD or both, PMN were stimulated with phorbol 12-myristate 13-acetate. Superoxide production was measured by lucigenin-enhanced chemiluminescence.Results:  Following 24-hr culture with either 17-03B2-estradiol or TCDD, PMN superoxide production was significantly reduced, however, no such inhibition was observed when PMN were cultured with both estradiol and TCDD. Using antagonists, the estradiol and TCDD effects on PMN superoxide production was shown to be estrogen and aryl hydrocarbon receptor mediated.Conclusions:  Estradiol and TCDD influence PMN oxidative burst through receptor mediated events. Such altered PMN function may have profound effects upon the normal endometrial cycle.}, address = {Department of Microbiology and Immunology, Dartmouth Medical School, 1 Medical Center Drive, Lebanon, NH 03756, USA}, author = {Abrahams, Vikki M. and Collins, Jane E. and Wira, Charles R. and Fanger, Michael W. and Yeaman, Grant R.}, citeulike-article-id = {4085513}, citeulike-linkout-0 = {http://dx.doi.org/10.1046/j.8755-8920.2003.00111.x}, citeulike-linkout-1 = {http://www3.interscience.wiley.com/cgi-bin/abstract/118848473/ABSTRACT}, doi = {10.1046/j.8755-8920.2003.00111.x}, journal = {American Journal Of Reproductive Immunology}, keywords = {immunomodulation}, number = {6}, pages = {463--472}, posted-at = {2009-02-23 13:41:10}, priority = {2}, title = {Inhibition of Human Polymorphonuclear Cell Oxidative Burst by 17-\&\#x03B2;-estradiol and 2,3,7,8-tetrachlorodibenzo-<i>p</i>-dioxin}, url = {http://dx.doi.org/10.1046/j.8755-8920.2003.00111.x}, volume = {50}, year = {2003} }

TY - JOUR ID - citeulike:4085513 L3 - citeulike-article-id:4085513 N2 - Abrahams VM, Collins JE, Wira CR, Fanger MW, Yeaman GR. Inhibition of human polymorphonuclear cell oxidative burst by 17-03B2-estradiol and 2,3,7,8-tetrachlorodibenzo-p-dioxin. AJRI 2003; 50:463ndash;472 © Blackwell Munksgaard, 2003Problem:  Polymorphonuclear cell (PMN) function may be directly influenced by 17-03B2-estradiol and the endocrine disruptor, 2,3,7,8-tetrachloro-dibenzo-p-dioxin (TCDD). This may have significant consequences on PMN function within the female reproductive tract. This study evaluated the effects of 17-03B2-estradiol and TCDD on PMN oxidative burst.Method of study:  Peripheral blood PMN were isolated from normal male donors. Following treatment with 17-03B2-estradiol, TCDD or both, PMN were stimulated with phorbol 12-myristate 13-acetate. Superoxide production was measured by lucigenin-enhanced chemiluminescence.Results:  Following 24-hr culture with either 17-03B2-estradiol or TCDD, PMN superoxide production was significantly reduced, however, no such inhibition was observed when PMN were cultured with both estradiol and TCDD. Using antagonists, the estradiol and TCDD effects on PMN superoxide production was shown to be estrogen and aryl hydrocarbon receptor mediated.Conclusions:  Estradiol and TCDD influence PMN oxidative burst through receptor mediated events. Such altered PMN function may have profound effects upon the normal endometrial cycle. IS - 6 JF - American Journal Of Reproductive Immunology AD - Department of Microbiology and Immunology, Dartmouth Medical School, 1 Medical Center Drive, Lebanon, NH 03756, USA EP - 472 TI - Inhibition of Human Polymorphonuclear Cell Oxidative Burst by 17-&#x03B2;-estradiol and 2,3,7,8-tetrachlorodibenzo-<i>p</i>-dioxin VL - 50 SP - 463 KW - immunomodulation AU - Abrahams, Vikki AU - Collins, Jane AU - Wira, Charles AU - Fanger, Michael AU - Yeaman, Grant PY - 2003/// UR - http://dx.doi.org/10.1046/j.8755-8920.2003.00111.x ER -