Membrane estrogen receptors identified by multiple antibody labeling and impeded-ligand binding. Export

@article{citeulike:4890616, abstract = {GH3/B6 rat pituitary tumor cells exhibit rapid prolactin release (within 5 min) when treated with nanomolar amounts of estrogen. However, the putative protein mediator of this nongenomic action has not been described. Using antibodies directed against a peptide representing the hinge region of the intracellular estrogen receptor (iER), we have demonstrated that these cells contain a membrane ER (mER). We now report that confocal scanning laser microscopy of cells labeled live with the anti-peptide antibody further supports a membrane localization of ER. The monoclonal antibodies H226 and H222 and a polyclonal antibody, ER21, each recognizing a unique epitope on iER (NH2 terminal to the DNA-binding region, within the steroid binding region, and the NH2-terminal end, respectively), also immunohistochemically label membrane proteins of immuno-selected GH3/B6 cells. These cells also specifically bind a fluorescent estrogen-BSA conjugate. Coincubation of cells with anti-ER antibody and the fluorescent estrogen-BSA conjugate reveals that these labels colocalize on cells. These results suggest that mER may be structurally similar to iER.}, author = {Pappas, T. C. and Gametchu, B. and Watson, C. S.}, citeulike-article-id = {4890616}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/7896011}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=7896011}, issn = {0892-6638}, journal = {The FASEB journal : official publication of the Federation of American Societies for Experimental Biology}, keywords = {estrogen, receptor, superlist, thelist}, month = {March}, number = {5}, pages = {404--410}, posted-at = {2009-06-18 11:15:47}, priority = {2}, title = {Membrane estrogen receptors identified by multiple antibody labeling and impeded-ligand binding.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/7896011}, volume = {9}, year = {1995} }

TY - JOUR ID - citeulike:4890616 L3 - citeulike-article-id:4890616 N2 - GH3/B6 rat pituitary tumor cells exhibit rapid prolactin release (within 5 min) when treated with nanomolar amounts of estrogen. However, the putative protein mediator of this nongenomic action has not been described. Using antibodies directed against a peptide representing the hinge region of the intracellular estrogen receptor (iER), we have demonstrated that these cells contain a membrane ER (mER). We now report that confocal scanning laser microscopy of cells labeled live with the anti-peptide antibody further supports a membrane localization of ER. The monoclonal antibodies H226 and H222 and a polyclonal antibody, ER21, each recognizing a unique epitope on iER (NH2 terminal to the DNA-binding region, within the steroid binding region, and the NH2-terminal end, respectively), also immunohistochemically label membrane proteins of immuno-selected GH3/B6 cells. These cells also specifically bind a fluorescent estrogen-BSA conjugate. Coincubation of cells with anti-ER antibody and the fluorescent estrogen-BSA conjugate reveals that these labels colocalize on cells. These results suggest that mER may be structurally similar to iER. IS - 5 JF - The FASEB journal : official publication of the Federation of American Societies for Experimental Biology SN - 0892-6638 EP - 410 TI - Membrane estrogen receptors identified by multiple antibody labeling and impeded-ligand binding. VL - 9 SP - 404 KW - estrogen KW - receptor KW - superlist KW - thelist AU - Pappas, TC AU - Gametchu, B AU - Watson, CS PY - 1995/03// UR - http://view.ncbi.nlm.nih.gov/pubmed/7896011 ER -