Reliability of follicle-stimulating hormone measurements in serum. Export

@article{citeulike:5271496, abstract = {BACKGROUND: Follicle-stimulating hormone (FSH), a member of gonadotropin family, is critical for follicular maturation and ovarian steroidogenesis. Serum FSH levels are known to fluctuate during different phases of menstrual cycle in premenopausal women, and increase considerably after the menopause as a result of ovarian function cessation. There is little existing evidence to guide researchers in estimating the reliability of serum FSH measurements. The objective of this study was to assess the reliability of FSH measurement using stored sera from an ongoing prospective cohort--the NYU Women's Health Study. METHODS: Sixty healthy women (16 premenopausal, 44 postmenopausal), who donated at least two blood samples at approximately 1-year intervals were studied. An immunoradiometric assay using a sandwich monoclonal antibodies technique was used to measure FSH levels in serum. RESULTS: The reliability of a single log-transformed FSH measurement, as determined by the intraclass correlation coefficient, was 0.70 for postmenopausal women (95\% confidence interval (CI), 0.55-0.82) and 0.09 for premenopausal women (95\% CI, 0-0.54). CONCLUSIONS: These results suggest that a single measurement is sufficient to characterize the serum FSH level in postmenopausal women and could be a useful tool in epidemiological research. For premenopausal women, however, the reliability coefficient was low, suggesting that a single determination is insufficient to reliably estimate a woman's true average serum FSH level and repeated measurements are desirable.}, author = {Arslan, Alan A. and Zeleniuch-Jacquotte, Anne and Lukanova, Annekatrin and Rinaldi, Sabina and Kaaks, Rudolf and Toniolo, Paolo}, citeulike-article-id = {5271496}, citeulike-linkout-0 = {http://dx.doi.org/10.1186/1477-7827-1-49}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/12844363}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=12844363}, day = {18}, doi = {10.1186/1477-7827-1-49}, issn = {1477-7827}, journal = {Reproductive Biology and Endocrinology}, keywords = {estrogen, measurement, reliability, superlist}, month = {June}, number = {1}, pages = {49+}, posted-at = {2009-07-26 13:55:58}, priority = {2}, title = {Reliability of follicle-stimulating hormone measurements in serum.}, url = {http://dx.doi.org/10.1186/1477-7827-1-49}, volume = {1}, year = {2003} }

TY - JOUR ID - citeulike:5271496 L3 - citeulike-article-id:5271496 N2 - BACKGROUND: Follicle-stimulating hormone (FSH), a member of gonadotropin family, is critical for follicular maturation and ovarian steroidogenesis. Serum FSH levels are known to fluctuate during different phases of menstrual cycle in premenopausal women, and increase considerably after the menopause as a result of ovarian function cessation. There is little existing evidence to guide researchers in estimating the reliability of serum FSH measurements. The objective of this study was to assess the reliability of FSH measurement using stored sera from an ongoing prospective cohort--the NYU Women's Health Study. METHODS: Sixty healthy women (16 premenopausal, 44 postmenopausal), who donated at least two blood samples at approximately 1-year intervals were studied. An immunoradiometric assay using a sandwich monoclonal antibodies technique was used to measure FSH levels in serum. RESULTS: The reliability of a single log-transformed FSH measurement, as determined by the intraclass correlation coefficient, was 0.70 for postmenopausal women (95% confidence interval (CI), 0.55-0.82) and 0.09 for premenopausal women (95% CI, 0-0.54). CONCLUSIONS: These results suggest that a single measurement is sufficient to characterize the serum FSH level in postmenopausal women and could be a useful tool in epidemiological research. For premenopausal women, however, the reliability coefficient was low, suggesting that a single determination is insufficient to reliably estimate a woman's true average serum FSH level and repeated measurements are desirable. IS - 1 JF - Reproductive Biology and Endocrinology SN - 1477-7827 TI - Reliability of follicle-stimulating hormone measurements in serum. VL - 1 SP - 49 KW - estrogen KW - measurement KW - reliability KW - superlist AU - Arslan, Alan AU - Zeleniuch-Jacquotte, Anne AU - Lukanova, Annekatrin AU - Rinaldi, Sabina AU - Kaaks, Rudolf AU - Toniolo, Paolo PY - 2003/06/18/ UR - http://dx.doi.org/10.1186/1477-7827-1-49 ER -