Cells keep a memory of their tissue origin during axolotl limb regeneration. Export

@article{citeulike:5036504, abstract = {During limb regeneration adult tissue is converted into a zone of undifferentiated progenitors called the blastema that reforms the diverse tissues of the limb. Previous experiments have led to wide acceptance that limb tissues dedifferentiate to form pluripotent cells. Here we have reexamined this question using an integrated GFP transgene to track the major limb tissues during limb regeneration in the salamander Ambystoma mexicanum (the axolotl). Surprisingly, we find that each tissue produces progenitor cells with restricted potential. Therefore, the blastema is a heterogeneous collection of restricted progenitor cells. On the basis of these findings, we further demonstrate that positional identity is a cell-type-specific property of blastema cells, in which cartilage-derived blastema cells harbour positional identity but Schwann-derived cells do not. Our results show that the complex phenomenon of limb regeneration can be achieved without complete dedifferentiation to a pluripotent state, a conclusion with important implications for regenerative medicine.}, author = {Kragl, Martin and Knapp, Dunja and Nacu, Eugen and Khattak, Shahryar and Maden, Malcolm and Epperlein, Hans Henning H. and Tanaka, Elly M.}, citeulike-article-id = {5036504}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/nature08152}, citeulike-linkout-1 = {http://dx.doi.org/10.1038/nature08152}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19571878}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19571878}, day = {2}, doi = {10.1038/nature08152}, issn = {1476-4687}, journal = {Nature}, keywords = {cell, regeneration, repair, salamander, stem}, month = {July}, number = {7251}, pages = {60--65}, posted-at = {2009-07-01 21:54:16}, priority = {2}, publisher = {Macmillan Publishers Limited. All rights reserved}, title = {Cells keep a memory of their tissue origin during axolotl limb regeneration.}, url = {http://dx.doi.org/10.1038/nature08152}, volume = {460}, year = {2009} }

TY - JOUR ID - citeulike:5036504 L3 - citeulike-article-id:5036504 N2 - During limb regeneration adult tissue is converted into a zone of undifferentiated progenitors called the blastema that reforms the diverse tissues of the limb. Previous experiments have led to wide acceptance that limb tissues dedifferentiate to form pluripotent cells. Here we have reexamined this question using an integrated GFP transgene to track the major limb tissues during limb regeneration in the salamander Ambystoma mexicanum (the axolotl). Surprisingly, we find that each tissue produces progenitor cells with restricted potential. Therefore, the blastema is a heterogeneous collection of restricted progenitor cells. On the basis of these findings, we further demonstrate that positional identity is a cell-type-specific property of blastema cells, in which cartilage-derived blastema cells harbour positional identity but Schwann-derived cells do not. Our results show that the complex phenomenon of limb regeneration can be achieved without complete dedifferentiation to a pluripotent state, a conclusion with important implications for regenerative medicine. IS - 7251 JF - Nature SN - 1476-4687 EP - 65 TI - Cells keep a memory of their tissue origin during axolotl limb regeneration. PB - Macmillan Publishers Limited. All rights reserved VL - 460 SP - 60 KW - cell KW - regeneration KW - repair KW - salamander KW - stem AU - Kragl, Martin AU - Knapp, Dunja AU - Nacu, Eugen AU - Khattak, Shahryar AU - Maden, Malcolm AU - Epperlein, Hans Henning AU - Tanaka, Elly PY - 2009/07/02/ UR - http://dx.doi.org/10.1038/nature08152 ER -