Role of Protein Kinase C, G-Protein Coupled Receptors, and Calcium Flux During Metamorphosis of the Sea Urchin Strongylocentrotus purpuratus Export

@article{citeulike:1724375, abstract = {Artificial inducers have been used to study signal-transduction pathways involved in metamorphosis of some marine invertebrates. However, the transduction mechanisms for echinoderms have been less explored. In the present study, participation of protein kinase C (PKC), G-protein-coupled receptors (GPCRs), and calcium has been investigated during metamorphosis of the sea urchin Stronglylocentrotus purpuratus. Competent larvae were induced with different drugs that activate (PKC and GP activators, Ca2+ ionophores, and inhibitors of Ca2+ ATPase) or inhibit (PKC, G-protein, and Ca2+ flux inhibitors) metamorphosis. Six of the compounds were effective: the PKC activators TPA and indolactam; the G-protein inhibitors suramin and guanosine; the calcium ionophore A23187, and the calcium ATPase inhibitor thapsigargin. TPA was effective at 0.001 {micro}M; indolactam was effective at 0.001 {micro}M. In the presence of KCl as inducer, the G-protein inhibitor suramin was effective at 10 {micro}M and guanosine at 0.001 {micro}M. In the presence of a bacterial film as inducer, suramin was effective at 50 {micro}M, and guanosine inhibited metamorphosis at 1 {micro}M. A23187 was effective at 5 and 10 {micro}M and thapsigargin at 50 and 100 {micro}M. Our results indicate that GPCRs, protein kinase C, and calcium participate in the metamorphosis of S. purpuratus. These elements of the transduction pathways triggered during metamorphosis may be part of a cascade of signal transduction routes that interact from induction to the end of the morphogenetic events that shape the postlarval form. In addition, according to the results obtained with G-protein inhibitors, the GPCRs may be shared between the artificial (KCl) and natural (biofilm) inducers.}, author = {Amador-Cano, G. and Carpizo-Ituarte, E. and Cristino-Jorge, D.}, citeulike-article-id = {1724375}, citeulike-linkout-0 = {http://www.biolbull.org/cgi/content/abstract/210/2/121}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/16641517}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=16641517}, day = {1}, journal = {Biol Bull}, keywords = {calcium, chemosensory, gpcr, inhibitors, metamorphosis, pkc, urchin}, month = {April}, number = {2}, pages = {121--131}, posted-at = {2007-10-03 15:55:22}, priority = {4}, title = {Role of Protein Kinase C, G-Protein Coupled Receptors, and Calcium Flux During Metamorphosis of the Sea Urchin Strongylocentrotus purpuratus}, url = {http://www.biolbull.org/cgi/content/abstract/210/2/121}, volume = {210}, year = {2006} }

TY - JOUR ID - citeulike:1724375 L3 - citeulike-article-id:1724375 N2 - Artificial inducers have been used to study signal-transduction pathways involved in metamorphosis of some marine invertebrates. However, the transduction mechanisms for echinoderms have been less explored. In the present study, participation of protein kinase C (PKC), G-protein-coupled receptors (GPCRs), and calcium has been investigated during metamorphosis of the sea urchin Stronglylocentrotus purpuratus. Competent larvae were induced with different drugs that activate (PKC and GP activators, Ca2+ ionophores, and inhibitors of Ca2+ ATPase) or inhibit (PKC, G-protein, and Ca2+ flux inhibitors) metamorphosis. Six of the compounds were effective: the PKC activators TPA and indolactam; the G-protein inhibitors suramin and guanosine; the calcium ionophore A23187, and the calcium ATPase inhibitor thapsigargin. TPA was effective at 0.001 {micro}M; indolactam was effective at 0.001 {micro}M. In the presence of KCl as inducer, the G-protein inhibitor suramin was effective at 10 {micro}M and guanosine at 0.001 {micro}M. In the presence of a bacterial film as inducer, suramin was effective at 50 {micro}M, and guanosine inhibited metamorphosis at 1 {micro}M. A23187 was effective at 5 and 10 {micro}M and thapsigargin at 50 and 100 {micro}M. Our results indicate that GPCRs, protein kinase C, and calcium participate in the metamorphosis of S. purpuratus. These elements of the transduction pathways triggered during metamorphosis may be part of a cascade of signal transduction routes that interact from induction to the end of the morphogenetic events that shape the postlarval form. In addition, according to the results obtained with G-protein inhibitors, the GPCRs may be shared between the artificial (KCl) and natural (biofilm) inducers. IS - 2 JF - Biol Bull EP - 131 TI - Role of Protein Kinase C, G-Protein Coupled Receptors, and Calcium Flux During Metamorphosis of the Sea Urchin Strongylocentrotus purpuratus VL - 210 SP - 121 KW - calcium KW - chemosensory KW - gpcr KW - inhibitors KW - metamorphosis KW - pkc KW - urchin AU - Amador-Cano, G AU - Carpizo-Ituarte, E AU - Cristino-Jorge, D PY - 2006/04/01/ UR - http://www.biolbull.org/cgi/content/abstract/210/2/121 ER -