An alpha2-macroglobulin-like protein is the cue to gregarious settlement of the barnacle Balanus amphitrite Export

@article{citeulike:1776157, abstract = {Many benthic marine invertebrates, like barnacles, have a planktonic larval stage whose primary purpose is dispersal. How these species colonize suitable substrata is fundamental to understanding their evolution, population biology, and wider community dynamics. Unlike larval dispersal, settlement occurs on a relatively small spatial scale and involves larval behavior in response to physical and chemical characteristics of the substratum. Biogenic chemical cues have been implicated in this process. Their identification, however, has proven challenging, no more so than for the chemical basis of barnacle gregariousness, which was first described >50 years ago. We now report that a biological cue to gregarious settlement, the settlement-inducing protein complex (SIPC), of the major fouling barnacle Balanus amphitrite is a previously undescribed glycoprotein. The SIPC shares a 30\% sequence homology with the thioester-containing family of proteins that includes the {alpha}2-macroglobulins. The cDNA (5.2 kb) of the SIPC encodes a protein precursor comprising 1,547 aa with a 17-residue signal peptide region. A number of structural characteristics and the absence of a thioester bond in the SIPC suggest that this molecule is a previously undescribed protein that may have evolved by duplication from an ancestral {alpha}2-macroglobulin gene. Although the SIPC is regarded as an adult cue that is recognized by the cyprid at settlement, it is also expressed in the juvenile and in larvae, where it may function in larva-larva settlement interactions. 10.1073/pnas.0602763103}, author = {Dreanno, Catherine and Matsumura, Kiyotaka and Dohmae, Naoshi and Takio, Koji and Hirota, Hiroshi and Kirby, Richard R. and Clare, Anthony S.}, citeulike-article-id = {1776157}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.0602763103}, citeulike-linkout-1 = {http://www.pnas.org/cgi/content/abstract/103/39/14396}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/16983086}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=16983086}, day = {26}, doi = {10.1073/pnas.0602763103}, journal = {Proceedings of the National Academy of Sciences}, keywords = {barnacle, chemosensory, cue, cyprid, glycoprotein, gregarious, macroglobulin, metamorphosis, settlement}, month = {September}, number = {39}, pages = {14396--14401}, posted-at = {2007-10-16 20:52:16}, priority = {3}, title = {An {alpha}2-macroglobulin-like protein is the cue to gregarious settlement of the barnacle Balanus amphitrite}, url = {http://dx.doi.org/10.1073/pnas.0602763103}, volume = {103}, year = {2006} }

TY - JOUR ID - citeulike:1776157 L3 - citeulike-article-id:1776157 N2 - Many benthic marine invertebrates, like barnacles, have a planktonic larval stage whose primary purpose is dispersal. How these species colonize suitable substrata is fundamental to understanding their evolution, population biology, and wider community dynamics. Unlike larval dispersal, settlement occurs on a relatively small spatial scale and involves larval behavior in response to physical and chemical characteristics of the substratum. Biogenic chemical cues have been implicated in this process. Their identification, however, has proven challenging, no more so than for the chemical basis of barnacle gregariousness, which was first described >50 years ago. We now report that a biological cue to gregarious settlement, the settlement-inducing protein complex (SIPC), of the major fouling barnacle Balanus amphitrite is a previously undescribed glycoprotein. The SIPC shares a 30% sequence homology with the thioester-containing family of proteins that includes the {alpha}2-macroglobulins. The cDNA (5.2 kb) of the SIPC encodes a protein precursor comprising 1,547 aa with a 17-residue signal peptide region. A number of structural characteristics and the absence of a thioester bond in the SIPC suggest that this molecule is a previously undescribed protein that may have evolved by duplication from an ancestral {alpha}2-macroglobulin gene. Although the SIPC is regarded as an adult cue that is recognized by the cyprid at settlement, it is also expressed in the juvenile and in larvae, where it may function in larva-larva settlement interactions. 10.1073/pnas.0602763103 IS - 39 JF - Proceedings of the National Academy of Sciences EP - 14401 TI - An {alpha}2-macroglobulin-like protein is the cue to gregarious settlement of the barnacle Balanus amphitrite VL - 103 SP - 14396 KW - barnacle KW - chemosensory KW - cue KW - cyprid KW - glycoprotein KW - gregarious KW - macroglobulin KW - metamorphosis KW - settlement AU - Dreanno, Catherine AU - Matsumura, Kiyotaka AU - Dohmae, Naoshi AU - Takio, Koji AU - Hirota, Hiroshi AU - Kirby, Richard AU - Clare, Anthony PY - 2006/09/26/ UR - http://dx.doi.org/10.1073/pnas.0602763103 ER -