A homologue of <I>snail</I> is expressed transiently in subsets of mesenchyme cells in the sea urchin embryo and is down-regulated in axis-deficient embryos Export

@article{citeulike:1875400, abstract = {Vertebrate members of the zinc finger transcription factor family related to Drosophila snail are expressed in neural crest and paraxial mesoderm along the left-right axis of the embryo. As simple deuterostomes, echinoderms are an important sister phylum for the chordates. We have identified populations of patterned, nonskeletogenic mesenchyme in the sea urchin Lytechinus variegatus by their expression of a sea urchin member of the snail family (Lv-snail). Lv-snail mRNA and protein are detectable at the midgastrula stage within the archenteron. At the late gastrula stage, a contiguous cluster of cells on the left side of the tip of the archenteron is Lv-snail-positive. At the early prism stage, two small clusters of mesenchyme cells near the presumptive arm buds are also Lv-snail-positive. At the pluteus stage, staining is detectable in isolated mesenchyme cells and the ciliated band. Based on fate mapping of secondary mesenchyme cells (SMCs) and double-label immunostaining, these patterns are consistent with expression of SNAIL by novel subsets of SMCs that are largely distinct from skeletogenic mesenchyme. In radialized embryos lacking normal bilateral symmetry, mesenchymal expression of Lv-SNAIL is abolished. These results suggest that transient expression of Lv-snail may be important for the differentiation of a subset of axially patterned nonskeletogenic mesenchyme cells and suggest conserved functions for snail family members in deuterostome development. Developmental Dynamics 235:3121-3131, 2006. {\copyright} 2006 Wiley-Liss, Inc.}, address = {Department of Zoology, University of Wisconsin-Madison, Madison, Wisconsin; Program in Cellular and Molecular Biology, University of Wisconsin-Madison, Madison, Wisconsin}, author = {Hardin, Jeff and Illingworth, Charles A.}, citeulike-article-id = {1875400}, citeulike-linkout-0 = {http://dx.doi.org/10.1002/dvdy.20941}, citeulike-linkout-1 = {http://www3.interscience.wiley.com/cgi-bin/abstract/112780091/ABSTRACT}, doi = {10.1002/dvdy.20941}, journal = {Developmental Dynamics}, keywords = {arms, ciliary, smc, transcription, urchin}, number = {11}, pages = {3121--3131}, posted-at = {2007-11-06 22:18:57}, priority = {2}, title = {A homologue of <I>snail</I> is expressed transiently in subsets of mesenchyme cells in the sea urchin embryo and is down-regulated in axis-deficient embryos}, url = {http://dx.doi.org/10.1002/dvdy.20941}, volume = {235}, year = {2006} }

TY - JOUR ID - citeulike:1875400 L3 - citeulike-article-id:1875400 N2 - Vertebrate members of the zinc finger transcription factor family related to Drosophila snail are expressed in neural crest and paraxial mesoderm along the left-right axis of the embryo. As simple deuterostomes, echinoderms are an important sister phylum for the chordates. We have identified populations of patterned, nonskeletogenic mesenchyme in the sea urchin Lytechinus variegatus by their expression of a sea urchin member of the snail family (Lv-snail). Lv-snail mRNA and protein are detectable at the midgastrula stage within the archenteron. At the late gastrula stage, a contiguous cluster of cells on the left side of the tip of the archenteron is Lv-snail-positive. At the early prism stage, two small clusters of mesenchyme cells near the presumptive arm buds are also Lv-snail-positive. At the pluteus stage, staining is detectable in isolated mesenchyme cells and the ciliated band. Based on fate mapping of secondary mesenchyme cells (SMCs) and double-label immunostaining, these patterns are consistent with expression of SNAIL by novel subsets of SMCs that are largely distinct from skeletogenic mesenchyme. In radialized embryos lacking normal bilateral symmetry, mesenchymal expression of Lv-SNAIL is abolished. These results suggest that transient expression of Lv-snail may be important for the differentiation of a subset of axially patterned nonskeletogenic mesenchyme cells and suggest conserved functions for snail family members in deuterostome development. Developmental Dynamics 235:3121-3131, 2006. © 2006 Wiley-Liss, Inc. IS - 11 JF - Developmental Dynamics AD - Department of Zoology, University of Wisconsin-Madison, Madison, Wisconsin; Program in Cellular and Molecular Biology, University of Wisconsin-Madison, Madison, Wisconsin EP - 3131 TI - A homologue of <I>snail</I> is expressed transiently in subsets of mesenchyme cells in the sea urchin embryo and is down-regulated in axis-deficient embryos VL - 235 SP - 3121 KW - arms KW - ciliary KW - smc KW - transcription KW - urchin AU - Hardin, Jeff AU - Illingworth, Charles PY - 2006/// UR - http://dx.doi.org/10.1002/dvdy.20941 ER -