Six3 functions in anterior neural plate specification by promoting cell proliferation and inhibiting Bmp4 expression Export

@article{citeulike:2480562, abstract = {Although it is well established that Six3 is a crucial regulator of vertebrate eye and forebrain development, it is unknown whether this homeodomain protein has a role in the initial specification of the anterior neural plate. In this study, we show that exogenous Six3 can expand the anterior neural plate in both Xenopus and zebrafish, and that this occurs in part through Six3-dependent transcriptional regulation of the cell cycle regulators cyclinD1 and p27Xic1, as well as the anti-neurogenic genes Zic2 and Xhairy2. However, Six3 can still expand the neural plate in the presence of cell cycle inhibitors and we show that this is likely to be due to its ability to repress the expression of Bmp4 in ectoderm adjacent to the anterior neural plate. Furthermore, exogenous Six3 is able to restore the size of the anterior neural plate in chordino mutant zebrafish, indicating that it has the ability to promote anterior neural development by antagonising the activity of the BMP pathway. On its own, Six3 is unable to induce neural tissue in animal caps, but it can do so in combination with Otx2. These results suggest a very early role for Six3 in specification of the anterior neural plate, through the regulation of cell proliferation and the inhibition of BMP signalling. 10.1242/dev.01814}, author = {Gestri, Gaia and Carl, Matthias and Appolloni, Irene and Wilson, Stephen W. and Barsacchi, Giuseppina and Andreazzoli, Massimiliano}, citeulike-article-id = {2480562}, citeulike-linkout-0 = {http://dx.doi.org/10.1242/dev.01814}, citeulike-linkout-1 = {http://dev.biologists.org/cgi/content/abstract/132/10/2401}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/15843413}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=15843413}, day = {15}, doi = {10.1242/dev.01814}, journal = {Development}, keywords = {bmp, danio, neural, proliferation, six3, specification, xenopus}, month = {May}, number = {10}, pages = {2401--2413}, posted-at = {2008-03-06 21:54:42}, priority = {2}, title = {Six3 functions in anterior neural plate specification by promoting cell proliferation and inhibiting Bmp4 expression}, url = {http://dx.doi.org/10.1242/dev.01814}, volume = {132}, year = {2005} }

TY - JOUR ID - citeulike:2480562 L3 - citeulike-article-id:2480562 N2 - Although it is well established that Six3 is a crucial regulator of vertebrate eye and forebrain development, it is unknown whether this homeodomain protein has a role in the initial specification of the anterior neural plate. In this study, we show that exogenous Six3 can expand the anterior neural plate in both Xenopus and zebrafish, and that this occurs in part through Six3-dependent transcriptional regulation of the cell cycle regulators cyclinD1 and p27Xic1, as well as the anti-neurogenic genes Zic2 and Xhairy2. However, Six3 can still expand the neural plate in the presence of cell cycle inhibitors and we show that this is likely to be due to its ability to repress the expression of Bmp4 in ectoderm adjacent to the anterior neural plate. Furthermore, exogenous Six3 is able to restore the size of the anterior neural plate in chordino mutant zebrafish, indicating that it has the ability to promote anterior neural development by antagonising the activity of the BMP pathway. On its own, Six3 is unable to induce neural tissue in animal caps, but it can do so in combination with Otx2. These results suggest a very early role for Six3 in specification of the anterior neural plate, through the regulation of cell proliferation and the inhibition of BMP signalling. 10.1242/dev.01814 IS - 10 JF - Development EP - 2413 TI - Six3 functions in anterior neural plate specification by promoting cell proliferation and inhibiting Bmp4 expression VL - 132 SP - 2401 KW - bmp KW - danio KW - neural KW - proliferation KW - six3 KW - specification KW - xenopus AU - Gestri, Gaia AU - Carl, Matthias AU - Appolloni, Irene AU - Wilson, Stephen AU - Barsacchi, Giuseppina AU - Andreazzoli, Massimiliano PY - 2005/05/15/ UR - http://dx.doi.org/10.1242/dev.01814 ER -