Bone blood flow and in vitro proliferation of bone marrow and trabecular bone osteoblast-like cells in ovariectomized rats.

@article{citeulike:3037926, abstract = {Ovariectomy in the rat induces a rapid osteopenia associated with an elevated bone turnover. One hundred and twenty-day-old rats were ovariectomized (OVX) or sham-operated (n = 6-8 per group and per time period studied). 45Ca accretion rate and bone blood flow (microspheres trapping technique) in the femurs were determined at 28, 42, 84, and 119 days after ovariectomy. Both parameters were markedly increased by 84 days and subsided thereafter. At the 42nd day, when bone turnover was maximal, bone marrow and trabecular bone cultures were obtained from sham-operated and ovariectomized animals (n = 10/group). Proliferation rate of bone marrow cells and trabecular osteoblast-like cells estimated by fibroblast colony-forming units (FCFU) efficiency and cell counting was markedly increased in primary and secondary cultures in ovariectomy. These data fitted well with the enhanced number of osteoblasts observed in situ in the long bone metaphyses of estrogen-depleted animals. As estrogens were shown in the literature to inhibit proliferation of the red cell line and of other hemopoietic lines, it is possible that estrogens, through a general mechanism, inhibit hemopoietic and stromal lines and also the proliferation of bone marrow-derived trabecular bone cells.}, address = {Cliniques universitaires de Bruxelles, Universit\'{e} libre de Bruxelles, Belgium.}, author = {Egrise, D. and Martin, D. and Neve, P. and Vienne, A. and Verhas, M. and Schoutens, A. }, citeulike-article-id = {3037926}, comment = {Google Scholar Citing Articles ( http://scholar.google.com/scholar?hl=en\&lr=\&client=firefox-a\&cites=4937647524493675111 ) }, issn = {0171-967X}, journal = {Calcified tissue international}, keywords = {osteoporosis\_serm, yr92}, month = {April}, number = {4}, pages = {336--341}, posted-at = {2008-07-24 00:03:25}, priority = {2}, title = {Bone blood flow and in vitro proliferation of bone marrow and trabecular bone osteoblast-like cells in ovariectomized rats.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/1571845}, volume = {50}, year = {1992} }

TY - JOUR ID - citeulike:3037926 L3 - citeulike-article-id:3037926 TI - Bone blood flow and in vitro proliferation of bone marrow and trabecular bone osteoblast-like cells in ovariectomized rats. JF - Calcified tissue international VL - 50 IS - 4 SP - 336 EP - 341 AD - Cliniques universitaires de Bruxelles, Université libre de Bruxelles, Belgium. SN - 0171-967X N2 - Ovariectomy in the rat induces a rapid osteopenia associated with an elevated bone turnover. One hundred and twenty-day-old rats were ovariectomized (OVX) or sham-operated (n = 6-8 per group and per time period studied). 45Ca accretion rate and bone blood flow (microspheres trapping technique) in the femurs were determined at 28, 42, 84, and 119 days after ovariectomy. Both parameters were markedly increased by 84 days and subsided thereafter. At the 42nd day, when bone turnover was maximal, bone marrow and trabecular bone cultures were obtained from sham-operated and ovariectomized animals (n = 10/group). Proliferation rate of bone marrow cells and trabecular osteoblast-like cells estimated by fibroblast colony-forming units (FCFU) efficiency and cell counting was markedly increased in primary and secondary cultures in ovariectomy. These data fitted well with the enhanced number of osteoblasts observed in situ in the long bone metaphyses of estrogen-depleted animals. As estrogens were shown in the literature to inhibit proliferation of the red cell line and of other hemopoietic lines, it is possible that estrogens, through a general mechanism, inhibit hemopoietic and stromal lines and also the proliferation of bone marrow-derived trabecular bone cells. KW - osteoporosis_serm KW - yr92 N1 - Google Scholar Citing Articles ( http://scholar.google.com/scholar?hl=en&lr=&client=firefox-a&cites=4937647524493675111 ) AU - Egrise, D AU - Martin, D AU - Neve, P AU - Vienne, A AU - Verhas, M AU - Schoutens, A PY - 1992/04// UR - http://view.ncbi.nlm.nih.gov/pubmed/1571845 ER -