Effects of Antioxidants on the Gamma-Radiation Damage of the Cultured Vascular Smooth Muscle Cells of Rat Aorta Export

@article{citeulike:5776781, abstract = {To study the protective effects of antioxidants on the radiation damages of the cells, vascular smooth muscle cells (VSMC) from thoracic aorta of Sprague-Dawley rats were cultured and irradiated with gamma-ray. Cell viability was measured by direct cell counting and MTT assay, and flow cytometry was performed to measure fractional distributions of the cells. Gamma-ray irradiation inhibited cell proliferations accompanied with decreased G1 phase and increased S- and G2/M phases, and the maximum effects were observed at 1500 or 2000 cGy. Submaximal concentrations of antioxidants, such as allopurinol, vitamin C, N-acetylcycteine (NAC), lipoic acid, dihydrolipoic acid and rebamipide tended to increase the cell viability suppressed by low dose of radiation (500 cGy), and enalapril and vitamin E increased it significantly. Allopurinol, vitamin E, NAC, lipoic acid, captopril and enalapril significantly increased G1 phase. Allopurinol and vitamin E tended to increase c-Myc expression, detected by Western blot, that was reduced by the radiation, and enalapril increased it significantly. The cell viability and c-Myc expression were highly correlated (r=0.97) with each other. These results suggest that antioxidants, especially enalapril and vitamin E, recover the viability of VSMC from gamma-radiation injury, through a mechanism which includes increase of c-Myc protein expression. KEY WORDS: Antioxidant, Vascular smooth muscle cell, Proliferation, Cell cycle, Gamma ray, c-Myc}, author = {Lee, Jong-Doo and Choi, Hyoung-Chul and Kang, Young-Jin and Kim, Myung-Se and Lee, Kwang-Youn}, citeulike-article-id = {5776781}, citeulike-linkout-0 = {http://www.koreamed.org/SearchBasic.php?DT=1&RID=417308}, citeulike-linkout-1 = {http://scholar.google.com/scholar?cluster=17464234330306649853}, citeulike-linkout-2 = {http://pdf.medrang.co.kr/paper/pdf/Kjpp/Kjpp011-05-05.pdf}, journal = {Korean J Physiol Pharmacol}, keywords = {aed, antioxidants, bio300, radioprotec, ros}, month = {October}, pages = {189--195}, posted-at = {2009-09-11 19:52:59}, priority = {2}, title = {Effects of Antioxidants on the Gamma-Radiation Damage of the Cultured Vascular Smooth Muscle Cells of Rat Aorta}, url = {http://www.koreamed.org/SearchBasic.php?DT=1&RID=417308}, volume = {11}, year = {2007} }

TY - JOUR ID - citeulike:5776781 L3 - citeulike-article-id:5776781 N2 - To study the protective effects of antioxidants on the radiation damages of the cells, vascular smooth muscle cells (VSMC) from thoracic aorta of Sprague-Dawley rats were cultured and irradiated with gamma-ray. Cell viability was measured by direct cell counting and MTT assay, and flow cytometry was performed to measure fractional distributions of the cells. Gamma-ray irradiation inhibited cell proliferations accompanied with decreased G1 phase and increased S- and G2/M phases, and the maximum effects were observed at 1500 or 2000 cGy. Submaximal concentrations of antioxidants, such as allopurinol, vitamin C, N-acetylcycteine (NAC), lipoic acid, dihydrolipoic acid and rebamipide tended to increase the cell viability suppressed by low dose of radiation (500 cGy), and enalapril and vitamin E increased it significantly. Allopurinol, vitamin E, NAC, lipoic acid, captopril and enalapril significantly increased G1 phase. Allopurinol and vitamin E tended to increase c-Myc expression, detected by Western blot, that was reduced by the radiation, and enalapril increased it significantly. The cell viability and c-Myc expression were highly correlated (r=0.97) with each other. These results suggest that antioxidants, especially enalapril and vitamin E, recover the viability of VSMC from gamma-radiation injury, through a mechanism which includes increase of c-Myc protein expression. KEY WORDS: Antioxidant, Vascular smooth muscle cell, Proliferation, Cell cycle, Gamma ray, c-Myc JF - Korean J Physiol Pharmacol EP - 195 TI - Effects of Antioxidants on the Gamma-Radiation Damage of the Cultured Vascular Smooth Muscle Cells of Rat Aorta VL - 11 SP - 189 KW - aed KW - antioxidants KW - bio300 KW - radioprotec KW - ros AU - Lee, Jong-Doo AU - Choi, Hyoung-Chul AU - Kang, Young-Jin AU - Kim, Myung-Se AU - Lee, Kwang-Youn PY - 2007/10// UR - http://www.koreamed.org/SearchBasic.php?DT=1&RID=417308 ER -