The calcium-sensing receptor regulates mammary gland parathyroid hormone-related protein production and calcium transport. Export

@article{citeulike:6043824, abstract = {The transfer of calcium from mother to milk during lactation is poorly understood. In this report, we demonstrate that parathyroid hormone-related protein (PTHrP) production and calcium transport in mammary epithelial cells are regulated by extracellular calcium acting through the calcium-sensing receptor (CaR). The CaR becomes expressed on mammary epithelial cells at the transition from pregnancy to lactation. Increasing concentrations of calcium, neomycin, and a calcimimetic compound suppress PTHrP secretion by mammary epithelial cells in vitro, whereas in vivo, systemic hypocalcemia increases PTHrP production, an effect that can be prevented by treatment with a calcimimetic. Hypocalcemia also reduces overall milk production and calcium content, while increasing milk osmolality and protein concentrations. The changes in milk calcium content, milk osmolality, and milk protein concentration were mitigated by calcimimetic infusions. Finally, in a three-dimensional culture system that recapitulates the lactating alveolus, activation of the basolateral CaR increases transcellular calcium transport independent of its effect on PTHrP. We conclude that the lactating mammary gland can sense calcium and adjusts its secretion of calcium, PTHrP, and perhaps water in response to changes in extracellular calcium concentration. We believe this defines a homeostatic system that helps to match milk production to the availability of calcium.}, author = {VanHouten, Joshua and Dann, Pamela and McGeoch, Grace and Brown, Edward M. and Krapcho, Karen and Neville, Margaret and Wysolmerski, John J.}, citeulike-article-id = {6043824}, citeulike-linkout-0 = {http://dx.doi.org/10.1172/JCI18776}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/14966569}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=14966569}, doi = {10.1172/JCI18776}, issn = {0021-9738}, journal = {The Journal of clinical investigation}, keywords = {calcium\_sensing\_receptor, car, pth}, month = {February}, number = {4}, pages = {598--608}, posted-at = {2009-10-30 18:23:01}, priority = {2}, title = {The calcium-sensing receptor regulates mammary gland parathyroid hormone-related protein production and calcium transport.}, url = {http://dx.doi.org/10.1172/JCI18776}, volume = {113}, year = {2004} }

TY - JOUR ID - citeulike:6043824 L3 - citeulike-article-id:6043824 N2 - The transfer of calcium from mother to milk during lactation is poorly understood. In this report, we demonstrate that parathyroid hormone-related protein (PTHrP) production and calcium transport in mammary epithelial cells are regulated by extracellular calcium acting through the calcium-sensing receptor (CaR). The CaR becomes expressed on mammary epithelial cells at the transition from pregnancy to lactation. Increasing concentrations of calcium, neomycin, and a calcimimetic compound suppress PTHrP secretion by mammary epithelial cells in vitro, whereas in vivo, systemic hypocalcemia increases PTHrP production, an effect that can be prevented by treatment with a calcimimetic. Hypocalcemia also reduces overall milk production and calcium content, while increasing milk osmolality and protein concentrations. The changes in milk calcium content, milk osmolality, and milk protein concentration were mitigated by calcimimetic infusions. Finally, in a three-dimensional culture system that recapitulates the lactating alveolus, activation of the basolateral CaR increases transcellular calcium transport independent of its effect on PTHrP. We conclude that the lactating mammary gland can sense calcium and adjusts its secretion of calcium, PTHrP, and perhaps water in response to changes in extracellular calcium concentration. We believe this defines a homeostatic system that helps to match milk production to the availability of calcium. IS - 4 JF - The Journal of clinical investigation SN - 0021-9738 EP - 608 TI - The calcium-sensing receptor regulates mammary gland parathyroid hormone-related protein production and calcium transport. VL - 113 SP - 598 KW - calcium_sensing_receptor KW - car KW - pth AU - VanHouten, Joshua AU - Dann, Pamela AU - McGeoch, Grace AU - Brown, Edward AU - Krapcho, Karen AU - Neville, Margaret AU - Wysolmerski, John PY - 2004/02// UR - http://dx.doi.org/10.1172/JCI18776 ER -