Induction of neurogenesis in the neocortex of adult mice. Export

@article{citeulike:418551, abstract = {Neurogenesis normally only occurs in limited areas of the adult mammalian brain--the hippocampus, olfactory bulb and epithelium, and at low levels in some regions of macaque cortex. Here we show that endogenous neural precursors can be induced in situ to differentiate into mature neurons, in regions of adult mammalian neocortex that do not normally undergo any neurogenesis. This differentiation occurs in a layer- and region-specific manner, and the neurons can re-form appropriate corticothalamic connections. We induced synchronous apoptotic degeneration of corticothalamic neurons in layer VI of anterior cortex of adult mice and examined the fates of dividing cells within cortex, using markers for DNA replication (5-bromodeoxyuridine; BrdU) and progressive neuronal differentiation. Newly made, BrdU-positive cells expressed NeuN, a mature neuronal marker, in regions of cortex undergoing targeted neuronal death and survived for at least 28 weeks. Subsets of BrdU+ precursors expressed Doublecortin, a protein found exclusively in migrating neurons, and Hu, an early neuronal marker. Retrograde labelling from thalamus demonstrated that BrdU+ neurons can form long-distance corticothalamic connections. Our results indicate that neuronal replacement therapies for neurodegenerative disease and CNS injury may be possible through manipulation of endogenous neural precursors in situ.}, address = {Division of Neuroscience, Children's Hospital, and Harvard Medical School, Boston, Massachusetts 02115, USA.}, author = {Magavi, S. S. and Leavitt, B. R. and Macklis, J. D.}, citeulike-article-id = {418551}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/35016083}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/10879536}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=10879536}, day = {22}, doi = {10.1038/35016083}, issn = {0028-0836}, journal = {Nature}, keywords = {ablation, adult, corticothalamicneurons, mouse, ng, photoactivation, svz}, month = {June}, number = {6789}, pages = {951--955}, posted-at = {2005-12-01 18:01:34}, priority = {2}, title = {Induction of neurogenesis in the neocortex of adult mice.}, url = {http://dx.doi.org/10.1038/35016083}, volume = {405}, year = {2000} }

TY - JOUR ID - citeulike:418551 L3 - citeulike-article-id:418551 N2 - Neurogenesis normally only occurs in limited areas of the adult mammalian brain--the hippocampus, olfactory bulb and epithelium, and at low levels in some regions of macaque cortex. Here we show that endogenous neural precursors can be induced in situ to differentiate into mature neurons, in regions of adult mammalian neocortex that do not normally undergo any neurogenesis. This differentiation occurs in a layer- and region-specific manner, and the neurons can re-form appropriate corticothalamic connections. We induced synchronous apoptotic degeneration of corticothalamic neurons in layer VI of anterior cortex of adult mice and examined the fates of dividing cells within cortex, using markers for DNA replication (5-bromodeoxyuridine; BrdU) and progressive neuronal differentiation. Newly made, BrdU-positive cells expressed NeuN, a mature neuronal marker, in regions of cortex undergoing targeted neuronal death and survived for at least 28 weeks. Subsets of BrdU+ precursors expressed Doublecortin, a protein found exclusively in migrating neurons, and Hu, an early neuronal marker. Retrograde labelling from thalamus demonstrated that BrdU+ neurons can form long-distance corticothalamic connections. Our results indicate that neuronal replacement therapies for neurodegenerative disease and CNS injury may be possible through manipulation of endogenous neural precursors in situ. IS - 6789 JF - Nature SN - 0028-0836 AD - Division of Neuroscience, Children's Hospital, and Harvard Medical School, Boston, Massachusetts 02115, USA. EP - 955 TI - Induction of neurogenesis in the neocortex of adult mice. VL - 405 SP - 951 KW - ablation KW - adult KW - corticothalamicneurons KW - mouse KW - ng KW - photoactivation KW - svz AU - Magavi, SS AU - Leavitt, BR AU - Macklis, JD PY - 2000/06/22/ UR - http://dx.doi.org/10.1038/35016083 ER -