Adult human subventricular, subgranular, and subpial zones contain astrocytes with a specialized intermediate filament cytoskeleton.

@article{citeulike:474302, abstract = {Human glial fibrillary acidic protein-delta (GFAP-delta) is a GFAP protein isoform that is encoded by an alternative splice variant of the GFAP-gene. As a result, GFAP-delta protein differs from the predominant splice form, GFAP-alpha, by its C-terminal protein sequence. In this study, we show that GFAP-delta protein is not expressed by all GFAP-expressing astrocytes but specifically by a subpopulation located in the subpial zone of the cerebral cortex, the subgranular zone of the hippocampus, and, most intensely, by a ribbon of astrocytes following the ependymal layer of the cerebral ventricles. Therefore, at least in the sub ventricular zone (SVZ), GFAP-delta specifically marks the population of astrocytes that contain the neural stem cells in the adult human brain. Interestingly, the SVZ astrocytes actively splice GFAP-delta transcripts, in contrast to astrocytes adjacent to this layer. Furthermore, we show that GFAP-delta protein, unlike GFAP-alpha, is not upregulated in astrogliosis. Our data therefore indicate a different functional role for GFAP-delta in astrocyte physiology. Finally, transfection studies showed that GFAP-delta protein expression has a negative effect on GFAP filament formation, and therefore could be important for modulating intermediate filament cytoskeletal properties, possibly facilitating astrocyte motility. Further studies on GFAP-delta and the cells that express it are important for gaining insights into its function during differentiation, migration and during health and disease.}, address = {Netherlands Institute for Brain Research, Graduate School Neurosciences, Amsterdam, the Netherlands.}, author = {Roelofs, R. F. and Fischer, D. F. and Houtman, S. H. and Sluijs, J. A. and Van Haren, W. and Van Leeuwen, F. W. and Hol, E. M.}, citeulike-article-id = {474302}, comment = {GFAP-delta in human nscs, not human astrocytes!}, doi = {10.1002/glia.20243}, issn = {0894-1491}, journal = {Glia}, keywords = {adult, dg, gfap, human, ng, svz}, month = {December}, number = {4}, pages = {289--300}, posted-at = {2006-01-21 21:00:37}, priority = {2}, title = {Adult human subventricular, subgranular, and subpial zones contain astrocytes with a specialized intermediate filament cytoskeleton.}, url = {http://dx.doi.org/10.1002/glia.20243}, volume = {52}, year = {2005} }

TY - JOUR ID - citeulike:474302 L3 - citeulike-article-id:474302 N2 - Human glial fibrillary acidic protein-delta (GFAP-delta) is a GFAP protein isoform that is encoded by an alternative splice variant of the GFAP-gene. As a result, GFAP-delta protein differs from the predominant splice form, GFAP-alpha, by its C-terminal protein sequence. In this study, we show that GFAP-delta protein is not expressed by all GFAP-expressing astrocytes but specifically by a subpopulation located in the subpial zone of the cerebral cortex, the subgranular zone of the hippocampus, and, most intensely, by a ribbon of astrocytes following the ependymal layer of the cerebral ventricles. Therefore, at least in the sub ventricular zone (SVZ), GFAP-delta specifically marks the population of astrocytes that contain the neural stem cells in the adult human brain. Interestingly, the SVZ astrocytes actively splice GFAP-delta transcripts, in contrast to astrocytes adjacent to this layer. Furthermore, we show that GFAP-delta protein, unlike GFAP-alpha, is not upregulated in astrogliosis. Our data therefore indicate a different functional role for GFAP-delta in astrocyte physiology. Finally, transfection studies showed that GFAP-delta protein expression has a negative effect on GFAP filament formation, and therefore could be important for modulating intermediate filament cytoskeletal properties, possibly facilitating astrocyte motility. Further studies on GFAP-delta and the cells that express it are important for gaining insights into its function during differentiation, migration and during health and disease. IS - 4 JF - Glia SN - 0894-1491 AD - Netherlands Institute for Brain Research, Graduate School Neurosciences, Amsterdam, the Netherlands. EP - 300 TI - Adult human subventricular, subgranular, and subpial zones contain astrocytes with a specialized intermediate filament cytoskeleton. VL - 52 SP - 289 KW - adult KW - dg KW - gfap KW - human KW - ng KW - svz N1 - GFAP-delta in human nscs, not human astrocytes! AU - Roelofs, RF AU - Fischer, DF AU - Houtman, SH AU - Sluijs, JA AU - Van Haren, W AU - Van Leeuwen, FW AU - Hol, EM PY - 2005/12// UR - http://dx.doi.org/10.1002/glia.20243 ER -