The ablation of glial fibrillary acidic protein-positive cells from the adult central nervous system results in the loss of forebrain neural stem cells but not retinal stem cells. Export

@article{citeulike:637265, abstract = {The adult mammalian forebrain subependyma contains neural stem cells (NSCs) capable of self-renewal and multilineage differentiation. The in vivo identification of NSCs has not been definitively addressed using a loss of function approach. Using a transgenic mouse expressing herpes-simplex virus thymidine kinase from the glial fibrillary acidic protein (GFAP) promotor, we have selectively killed dividing GFAP-positive cells in the presence of ganciclovir (GCV) and shown a > 95\% loss in the numbers of NSCs, as assayed by the formation of clonally derived neurospheres in vitro. This loss is seen following 3 days of GCV exposure in vivo or in vitro only and cannot be rescued by coculturing with pure astrocyte populations or control (green fluorescent protein-expressing) subependymal cells. Exposure to GCV in vitro has no effect on adult retinal stem cells hence, we conclude that adult forebrain NSCs comprise a subpopulation of the GFAP-positive cells within the subependyma.}, address = {Department of Surgery, University of Toronto, 1 King's College Circle, Toronto, Ontario M5S 1A8, Canada. cindi.morsehead@utoronto.ca}, author = {Morshead, C. M. and Garcia, A. D. and Sofroniew, M. V. and van Der Kooy, D.}, citeulike-article-id = {637265}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/12859339}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=12859339}, issn = {0953-816X}, journal = {Eur J Neurosci}, keywords = {ablation, adult, gfap-tk, mouse, ng}, month = {July}, number = {1}, pages = {76--84}, posted-at = {2006-05-16 14:53:07}, priority = {2}, title = {The ablation of glial fibrillary acidic protein-positive cells from the adult central nervous system results in the loss of forebrain neural stem cells but not retinal stem cells.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/12859339}, volume = {18}, year = {2003} }

TY - JOUR ID - citeulike:637265 L3 - citeulike-article-id:637265 N2 - The adult mammalian forebrain subependyma contains neural stem cells (NSCs) capable of self-renewal and multilineage differentiation. The in vivo identification of NSCs has not been definitively addressed using a loss of function approach. Using a transgenic mouse expressing herpes-simplex virus thymidine kinase from the glial fibrillary acidic protein (GFAP) promotor, we have selectively killed dividing GFAP-positive cells in the presence of ganciclovir (GCV) and shown a > 95% loss in the numbers of NSCs, as assayed by the formation of clonally derived neurospheres in vitro. This loss is seen following 3 days of GCV exposure in vivo or in vitro only and cannot be rescued by coculturing with pure astrocyte populations or control (green fluorescent protein-expressing) subependymal cells. Exposure to GCV in vitro has no effect on adult retinal stem cells hence, we conclude that adult forebrain NSCs comprise a subpopulation of the GFAP-positive cells within the subependyma. IS - 1 JF - Eur J Neurosci SN - 0953-816X AD - Department of Surgery, University of Toronto, 1 King's College Circle, Toronto, Ontario M5S 1A8, Canada. cindi.morsehead@utoronto.ca EP - 84 TI - The ablation of glial fibrillary acidic protein-positive cells from the adult central nervous system results in the loss of forebrain neural stem cells but not retinal stem cells. VL - 18 SP - 76 KW - ablation KW - adult KW - gfap-tk KW - mouse KW - ng AU - Morshead, CM AU - Garcia, AD AU - Sofroniew, MV AU - van Der Kooy, D PY - 2003/07// UR - http://view.ncbi.nlm.nih.gov/pubmed/12859339 ER -