Neurogenesis in the dentate gyrus of the adult rat: age-related decrease of neuronal progenitor proliferation Export

@article{citeulike:666583, abstract = {The hippocampus is one of the few areas of the rodent brain that continues to produce neurons postnatally. Neurogenesis reportedly persists in rats up to 11 months of age. Using bromodeoxyuridine (BrdU) labeling, the present study confirms that in the adult rat brain, neuronal progenitor cells divide at the border between the hilus and the granule cell layer (GCL). In adult rats, the progeny of these cells migrate into the GCL and express the neuronal markers NeuN and calbindin-D28k. However, neurogenesis was drastically reduced in aged rats. Six-to 27-month-old Fischer rats were injected intraperitoneally with BrdU to detect newborn cells in vivo and to follow their fate in the dentate gyrus. When killed 4-6 weeks after BrdU labeling, 12- to 27-month-old rats exhibited a significant decline in the density of BrdU-positive cells in the granule cell layer compared with 6-month-old controls. Decreased neurogenesis in aging rats was accompanied by reduced immunoreactivity for poly-sialylated neural cell adhesion molecule, a molecule that is involved in migration and process elongation of developing neurons. When animals were killed immediately (12 hr) after BrdU injection, significantly fewer labeled cells were observed in the GCL and adjacent subgranular zone of aged rats, indicative of a decrease in mitotic activity of neuronal precursor cells. The reduced proliferation was not attributable to a general aged-related metabolic impairment, because the density of BrdU-positive cells was not altered in other brain regions with known mitotic activity (e.g., hilus and lateral ventricle wall). The decline in neurogenesis that occurs throughout the lifespan of an animal can thus be related to a decreasing proliferation of granule cell precursors.}, address = {Laboratory of Genetics, Salk Institute, La Jolla, California 92037, USA.}, author = {Kuhn, H. G. and Dickinson-Anson, H. and Gage, F. H.}, citeulike-article-id = {666583}, citeulike-linkout-0 = {http://www.jneurosci.org/cgi/content/abstract/16/6/2027}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/8604047}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=8604047}, day = {15}, issn = {0270-6474}, journal = {J. Neurosci.}, keywords = {adult, age, dg, ng, rat}, month = {March}, number = {6}, pages = {2027--2033}, posted-at = {2006-05-23 22:04:07}, priority = {2}, title = {Neurogenesis in the dentate gyrus of the adult rat: age-related decrease of neuronal progenitor proliferation}, url = {http://www.jneurosci.org/cgi/content/abstract/16/6/2027}, volume = {16}, year = {1996} }

TY - JOUR ID - citeulike:666583 L3 - citeulike-article-id:666583 N2 - The hippocampus is one of the few areas of the rodent brain that continues to produce neurons postnatally. Neurogenesis reportedly persists in rats up to 11 months of age. Using bromodeoxyuridine (BrdU) labeling, the present study confirms that in the adult rat brain, neuronal progenitor cells divide at the border between the hilus and the granule cell layer (GCL). In adult rats, the progeny of these cells migrate into the GCL and express the neuronal markers NeuN and calbindin-D28k. However, neurogenesis was drastically reduced in aged rats. Six-to 27-month-old Fischer rats were injected intraperitoneally with BrdU to detect newborn cells in vivo and to follow their fate in the dentate gyrus. When killed 4-6 weeks after BrdU labeling, 12- to 27-month-old rats exhibited a significant decline in the density of BrdU-positive cells in the granule cell layer compared with 6-month-old controls. Decreased neurogenesis in aging rats was accompanied by reduced immunoreactivity for poly-sialylated neural cell adhesion molecule, a molecule that is involved in migration and process elongation of developing neurons. When animals were killed immediately (12 hr) after BrdU injection, significantly fewer labeled cells were observed in the GCL and adjacent subgranular zone of aged rats, indicative of a decrease in mitotic activity of neuronal precursor cells. The reduced proliferation was not attributable to a general aged-related metabolic impairment, because the density of BrdU-positive cells was not altered in other brain regions with known mitotic activity (e.g., hilus and lateral ventricle wall). The decline in neurogenesis that occurs throughout the lifespan of an animal can thus be related to a decreasing proliferation of granule cell precursors. IS - 6 JF - J. Neurosci. SN - 0270-6474 AD - Laboratory of Genetics, Salk Institute, La Jolla, California 92037, USA. EP - 2033 TI - Neurogenesis in the dentate gyrus of the adult rat: age-related decrease of neuronal progenitor proliferation VL - 16 SP - 2027 KW - adult KW - age KW - dg KW - ng KW - rat AU - Kuhn, HG AU - Dickinson-Anson, H AU - Gage, FH PY - 1996/03/15/ UR - http://www.jneurosci.org/cgi/content/abstract/16/6/2027 ER -