Genotypic characterization and species identification of Fasciola spp. with implications regarding the isolates infecting goats in Vietnam Export

@article{citeulike:5759199, abstract = {Ribosomal RNA sequences (361 or 362 bp) of the second internal transcribed spacer 2 (ITS-2) and a portion of mitochondrial cox1 (423 bp) for Fasciola spp. obtained from specimens collected in indigenous and hybrid goats and sheep in Vietnam were characterized for genotypic status and hybridization/introgression. Alignment of 48 ITS-2 sequences (also those from goats and sheep in this study) indicates that F. gigantica and F. hepatica differ typically from each other at seven sites whereas one of these is a distinguishing deletion (T) at the 327th position in F. gigantica relative to F. hepatica . The isolates from the mountainous goats in the North of Vietnam (Yen Bai province) showed the ITS-2 composition relatively identical to that of F. hepatica. The ITS-2 sequences from populations of Fasciola isolates in goats had probably experienced introgression/hybridization as reported previously in other ruminants and humans. All Vietnamese goat-of-origin specimens had high pairwise percentage of mitochondrial cox1 sequences to F. gigantica (97–100\%), and very low identity to F. hepatica (91–93\%), suggesting their maternal linkage to be traced to F. gigantica . The presence of hybrid and/or introgressed populations of liver flukes bearing genetic material from both F. hepatica and F. gigantica in the goats/sheep in Vietnam, regardless of indigenous or imported hosts, appears to be the first demonstration from a tropical country.}, author = {Nguyen, Thanh G. and Van De, Nguyen and Vercruysse, Jozef and Dorny, Pierre and Le, Thanh H.}, citeulike-article-id = {5759199}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.exppara.2009.09.001}, citeulike-linkout-1 = {http://linkinghub.elsevier.com/retrieve/pii/S0014489409002562}, day = {04}, doi = {10.1016/j.exppara.2009.09.001}, issn = {00144894}, journal = {Experimental Parasitology}, keywords = {fasciola-gigantica, fasciola-hepatica, goats, hybridization, internal-transcribed-spacer-2, introgression, vietnam}, month = {December}, number = {4}, pages = {354--361}, posted-at = {2009-10-27 09:31:42}, priority = {2}, title = {Genotypic characterization and species identification of Fasciola spp. with implications regarding the isolates infecting goats in Vietnam}, url = {http://dx.doi.org/10.1016/j.exppara.2009.09.001}, volume = {123}, year = {2009} }

TY - JOUR ID - citeulike:5759199 L3 - citeulike-article-id:5759199 N2 - Ribosomal RNA sequences (361 or 362 bp) of the second internal transcribed spacer 2 (ITS-2) and a portion of mitochondrial cox1 (423 bp) for Fasciola spp. obtained from specimens collected in indigenous and hybrid goats and sheep in Vietnam were characterized for genotypic status and hybridization/introgression. Alignment of 48 ITS-2 sequences (also those from goats and sheep in this study) indicates that F. gigantica and F. hepatica differ typically from each other at seven sites whereas one of these is a distinguishing deletion (T) at the 327th position in F. gigantica relative to F. hepatica . The isolates from the mountainous goats in the North of Vietnam (Yen Bai province) showed the ITS-2 composition relatively identical to that of F. hepatica. The ITS-2 sequences from populations of Fasciola isolates in goats had probably experienced introgression/hybridization as reported previously in other ruminants and humans. All Vietnamese goat-of-origin specimens had high pairwise percentage of mitochondrial cox1 sequences to F. gigantica (97–100%), and very low identity to F. hepatica (91–93%), suggesting their maternal linkage to be traced to F. gigantica . The presence of hybrid and/or introgressed populations of liver flukes bearing genetic material from both F. hepatica and F. gigantica in the goats/sheep in Vietnam, regardless of indigenous or imported hosts, appears to be the first demonstration from a tropical country. IS - 4 JF - Experimental Parasitology SN - 00144894 EP - 361 TI - Genotypic characterization and species identification of Fasciola spp. with implications regarding the isolates infecting goats in Vietnam VL - 123 SP - 354 KW - fasciola-gigantica KW - fasciola-hepatica KW - goats KW - hybridization KW - internal-transcribed-spacer-2 KW - introgression KW - vietnam AU - Nguyen, Thanh AU - Van De, Nguyen AU - Vercruysse, Jozef AU - Dorny, Pierre AU - Le, Thanh PY - 2009/12/04/ UR - http://dx.doi.org/10.1016/j.exppara.2009.09.001 ER -