Interactions of microbial biofilms with toxic trace metals: 1. Observation and modeling of cell growth, attachment, and production of extracellular polymer Export

@article{citeulike:5973441, abstract = {Adsorbent surfaces in natural and engineered systems are frequently modifies by bacterial attachment, growth of a biofilm, and bacterial production of extracellular polymer. Attached cells or sorbed polymers may alter the metal-binding characteristics of the supporting substratum and influence metal partitioning. The interdependent behavior of toxic trace metal partitioning and biofilm development requires description of the interaction between cell growth with its accompanying polymer production and metal speciation. In this article, the first of a two part series, a mechanistic model is developed to describe the growth of a film-forming bacterium which adheres to a substratum through the production of extracellular biopolymers. Each bacterial cell was modeled as a two-component structure consisting of active cell mass and biopolymer. The biopolymer component was further divided into cell-associated and dissolved categories to distinguish which remained naturally bound to cell surfaces from that which did not. Use of this structured model permitted independent description of the dynamics of cell growth, and polymer production, both of which may influence trace metal behavior. Employing parameters obtained from independent experiments as well as published values, the model satisfactorily predicts experimental observations of bacterial growth, attachment and detachment, biopolymer production, and adsorption of polymer onto solid (glass) surfaces. The model stimulated transient and steady-state biofilm systems equally well. In the second article in this series, we describe how this model may be extended and utilized to make predictions of the behavior of transient and steady-state biofilm systems in the presence of a toxic transition metal(Pb). {\copyright} 1994 John Wiley \& Sons, Inc.}, address = {School of Chemical Engineering, Cornell University, Ithaca, New York 14853; School of Civil and Environmental Engineering, Cornell University, Ithaca, New York 14853}, author = {Hsieh, Ke M. and Murgel, George A. and Lion, Leonard W. and Shuler, Michael L.}, citeulike-article-id = {5973441}, citeulike-linkout-0 = {http://dx.doi.org/10.1002/bit.260440211}, citeulike-linkout-1 = {http://www3.interscience.wiley.com/cgi-bin/abstract/107624171/ABSTRACT}, doi = {10.1002/bit.260440211}, issn = {1097-0290}, journal = {Biotechnology and Bioengineering}, number = {2}, pages = {219--231}, posted-at = {2009-10-20 10:23:39}, priority = {2}, title = {Interactions of microbial biofilms with toxic trace metals: 1. Observation and modeling of cell growth, attachment, and production of extracellular polymer}, url = {http://dx.doi.org/10.1002/bit.260440211}, volume = {44}, year = {1994} }

TY - JOUR ID - citeulike:5973441 L3 - citeulike-article-id:5973441 N2 - Adsorbent surfaces in natural and engineered systems are frequently modifies by bacterial attachment, growth of a biofilm, and bacterial production of extracellular polymer. Attached cells or sorbed polymers may alter the metal-binding characteristics of the supporting substratum and influence metal partitioning. The interdependent behavior of toxic trace metal partitioning and biofilm development requires description of the interaction between cell growth with its accompanying polymer production and metal speciation. In this article, the first of a two part series, a mechanistic model is developed to describe the growth of a film-forming bacterium which adheres to a substratum through the production of extracellular biopolymers. Each bacterial cell was modeled as a two-component structure consisting of active cell mass and biopolymer. The biopolymer component was further divided into cell-associated and dissolved categories to distinguish which remained naturally bound to cell surfaces from that which did not. Use of this structured model permitted independent description of the dynamics of cell growth, and polymer production, both of which may influence trace metal behavior. Employing parameters obtained from independent experiments as well as published values, the model satisfactorily predicts experimental observations of bacterial growth, attachment and detachment, biopolymer production, and adsorption of polymer onto solid (glass) surfaces. The model stimulated transient and steady-state biofilm systems equally well. In the second article in this series, we describe how this model may be extended and utilized to make predictions of the behavior of transient and steady-state biofilm systems in the presence of a toxic transition metal(Pb). © 1994 John Wiley & Sons, Inc. IS - 2 JF - Biotechnology and Bioengineering SN - 1097-0290 AD - School of Chemical Engineering, Cornell University, Ithaca, New York 14853; School of Civil and Environmental Engineering, Cornell University, Ithaca, New York 14853 EP - 231 TI - Interactions of microbial biofilms with toxic trace metals: 1. Observation and modeling of cell growth, attachment, and production of extracellular polymer VL - 44 SP - 219 AU - Hsieh, Ke AU - Murgel, George AU - Lion, Leonard AU - Shuler, Michael PY - 1994/// UR - http://dx.doi.org/10.1002/bit.260440211 ER -