High Mobility Group Protein-B1 Interacts with Sterol Regulatory Element-binding Proteins to Enhance Their DNA Binding Export

@article{citeulike:5704822, abstract = {Sterol regulatory element-binding proteins (SREBPs) are transcription factors that are predominately involved in the regulation of lipogenic and cholesterogenic enzyme gene expression. To identify unknown proteins that interact with SREBP, we screened nuclear extract proteins with 35S-labeled SREBP-1 bait in Far Western blotting analysis. Using this approach, high mobility group protein-B1 (HMGB1), a chromosomal protein, was identified as a novel SREBP interacting protein. In vitro glutathione S-transferase pull-down and in vivo coimmunoprecipitation studies confirmed an interaction between HMGB1 and both SREBP-1 and -2. The protein-protein interaction was mediated through the helix-loop-helix domain of SREBP-1, residues 309-344, and the A box of HMGB1. Furthermore, an electrophoretic mobility shift assay demonstrated that HMGB1 enhances SREBPs binding to their cognate DNA sequences. Moreover, luciferase reporter analyses, including RNA interference technique showed that HMGB1 potentiates the transcriptional activities of SREBP in cultured cells. These findings raise the intriguing possibility that HMGB1 is potentially involved in the regulation of lipogenic and cholesterogenic gene transcription. 10.1074/jbc.M414549200}, author = {Najima, Yuho and Yahagi, Naoya and Takeuchi, Yoshinori and Matsuzaka, Takashi and Sekiya, Motohiro and Nakagawa, Yoshimi and Amemiya-Kudo, Michiyo and Okazaki, Hiroaki and Okazaki, Sachiko and Tamura, Yoshiaki and Iizuka, Yoko and Ohashi, Ken and Harada, Kenji and Gotoda, Takanari and Nagai, Ryozo and Kadowaki, Takashi and Ishibashi, Shun and Yamada, Nobuhiro and Osuga, Jun-Ichi and Shimano, Hitoshi}, citeulike-article-id = {5704822}, citeulike-linkout-0 = {http://dx.doi.org/10.1074/jbc.M414549200}, citeulike-linkout-1 = {http://www.jbc.org/cgi/content/abstract/280/30/27523}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/16040616}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=16040616}, day = {29}, doi = {10.1074/jbc.M414549200}, journal = {J. Biol. Chem.}, keywords = {hmgb1, inflammation, lipogenesis, liver, obesity, srebp, srebp1c, steatosis}, month = {July}, number = {30}, pages = {27523--27532}, posted-at = {2009-09-02 00:44:09}, priority = {2}, title = {High Mobility Group Protein-B1 Interacts with Sterol Regulatory Element-binding Proteins to Enhance Their DNA Binding}, url = {http://dx.doi.org/10.1074/jbc.M414549200}, volume = {280}, year = {2005} }

TY - JOUR ID - citeulike:5704822 L3 - citeulike-article-id:5704822 N2 - Sterol regulatory element-binding proteins (SREBPs) are transcription factors that are predominately involved in the regulation of lipogenic and cholesterogenic enzyme gene expression. To identify unknown proteins that interact with SREBP, we screened nuclear extract proteins with 35S-labeled SREBP-1 bait in Far Western blotting analysis. Using this approach, high mobility group protein-B1 (HMGB1), a chromosomal protein, was identified as a novel SREBP interacting protein. In vitro glutathione S-transferase pull-down and in vivo coimmunoprecipitation studies confirmed an interaction between HMGB1 and both SREBP-1 and -2. The protein-protein interaction was mediated through the helix-loop-helix domain of SREBP-1, residues 309-344, and the A box of HMGB1. Furthermore, an electrophoretic mobility shift assay demonstrated that HMGB1 enhances SREBPs binding to their cognate DNA sequences. Moreover, luciferase reporter analyses, including RNA interference technique showed that HMGB1 potentiates the transcriptional activities of SREBP in cultured cells. These findings raise the intriguing possibility that HMGB1 is potentially involved in the regulation of lipogenic and cholesterogenic gene transcription. 10.1074/jbc.M414549200 IS - 30 JF - J. Biol. Chem. EP - 27532 TI - High Mobility Group Protein-B1 Interacts with Sterol Regulatory Element-binding Proteins to Enhance Their DNA Binding VL - 280 SP - 27523 KW - hmgb1 KW - inflammation KW - lipogenesis KW - liver KW - obesity KW - srebp KW - srebp1c KW - steatosis AU - Najima, Yuho AU - Yahagi, Naoya AU - Takeuchi, Yoshinori AU - Matsuzaka, Takashi AU - Sekiya, Motohiro AU - Nakagawa, Yoshimi AU - Amemiya-Kudo, Michiyo AU - Okazaki, Hiroaki AU - Okazaki, Sachiko AU - Tamura, Yoshiaki AU - Iizuka, Yoko AU - Ohashi, Ken AU - Harada, Kenji AU - Gotoda, Takanari AU - Nagai, Ryozo AU - Kadowaki, Takashi AU - Ishibashi, Shun AU - Yamada, Nobuhiro AU - Osuga, Jun-Ichi AU - Shimano, Hitoshi PY - 2005/07/29/ UR - http://dx.doi.org/10.1074/jbc.M414549200 ER -