Conjugative transfer and autonomous replication of a promiscuous IncQ plasmid in the cyanobacterium Synechocystis PCC 6803 Export

@article{citeulike:2874542, abstract = {The promiscuous IncQ plasmid pKT210 (Cmr, Smr) is efficiently transferred by transpecific conjugation from Escherichia coli to the facultatively heterotrophic cyanobacterium Synechocystis PCC6803 when mobilized by a helper plasmid coding for IncP transfer functions. The IncQ plasmid is stably maintained in the cyanobacterium as an autonomously replicating multicopy plasmid with no detectable structural alterations and can be recovered by transformation back to E. coli when using a mcrA mcrB host. Thus, the replicative host-range of IncQ plasmids extends beyond purple bacteria to the distinct procaryotic taxon of cyanobacteria, allowing the use of these small plasmids as convenient cloning vectors in Synechocystis PCC6803 and presumably also in cyanobacteria that are not amenable to genetic transformation. In contrast, an IncQ plasmid bearing the TRP1 gene of Saccharomyces cerevisiae failed to replicate when transferred to that yeast by transformation.}, author = {Kreps, Sabine and Ferino, Fabrice and Mosrin, Christine and Gerits, Jozef and Mergeay, Max and Thuriaux, Pierre}, citeulike-article-id = {2874542}, citeulike-linkout-0 = {http://dx.doi.org/10.1007/BF00280378}, day = {1}, doi = {10.1007/BF00280378}, journal = {Molecular and General Genetics MGG}, keywords = {pcc6803, plasmid, transformation}, month = {March}, number = {1}, pages = {129--133}, posted-at = {2008-06-09 08:49:40}, priority = {2}, title = {Conjugative transfer and autonomous replication of a promiscuous IncQ plasmid in the cyanobacterium Synechocystis PCC 6803}, url = {http://dx.doi.org/10.1007/BF00280378}, volume = {221}, year = {1990} }

TY - JOUR ID - citeulike:2874542 L3 - citeulike-article-id:2874542 N2 - The promiscuous IncQ plasmid pKT210 (Cmr, Smr) is efficiently transferred by transpecific conjugation from Escherichia coli to the facultatively heterotrophic cyanobacterium Synechocystis PCC6803 when mobilized by a helper plasmid coding for IncP transfer functions. The IncQ plasmid is stably maintained in the cyanobacterium as an autonomously replicating multicopy plasmid with no detectable structural alterations and can be recovered by transformation back to E. coli when using a mcrA mcrB host. Thus, the replicative host-range of IncQ plasmids extends beyond purple bacteria to the distinct procaryotic taxon of cyanobacteria, allowing the use of these small plasmids as convenient cloning vectors in Synechocystis PCC6803 and presumably also in cyanobacteria that are not amenable to genetic transformation. In contrast, an IncQ plasmid bearing the TRP1 gene of Saccharomyces cerevisiae failed to replicate when transferred to that yeast by transformation. IS - 1 JF - Molecular and General Genetics MGG EP - 133 TI - Conjugative transfer and autonomous replication of a promiscuous IncQ plasmid in the cyanobacterium Synechocystis PCC 6803 VL - 221 SP - 129 KW - pcc6803 KW - plasmid KW - transformation AU - Kreps, Sabine AU - Ferino, Fabrice AU - Mosrin, Christine AU - Gerits, Jozef AU - Mergeay, Max AU - Thuriaux, Pierre PY - 1990/03/01/ UR - http://dx.doi.org/10.1007/BF00280378 ER -