The multifunctional RNA-binding protein hnRNP A1 is required for processing of miR-18a. Export

@article{citeulike:1440426, abstract = {hnRNP A1 is an RNA-binding protein involved in various aspects of RNA processing. Use of an in vivo cross-linking and immunoprecipitation protocol to find hnRNP A1 RNA targets resulted in the identification of a microRNA (miRNA) precursor, pre-miR-18a. This microRNA is expressed as part of a cluster of intronic RNAs, including miR-17, miR-18a, miR-19a, miR-20a, miR-19b-1 and miR-92, and potentially acts as an oncogene. Here we show that hnRNP A1 binds specifically to the primary RNA sequence pri-miR-18a before Drosha processing. HeLa cells depleted of hnRNP A1 have reduced in vitro processing activity with pri-miR-18a and also show reduced abundances of endogenous pre-miR-18a. Furthermore, we show that hnRNP A1 is required for miR-18a-mediated repression of a target reporter in vivo. These results underscore a previously uncharacterized role for general RNA-binding proteins as auxiliary factors that facilitate the processing of specific miRNAs.}, author = {Guil, Sonia and C\'{a}ceres, Javier F.}, citeulike-article-id = {1440426}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/nsmb1250}, citeulike-linkout-1 = {http://dx.doi.org/10.1038/nsmb1250}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/17558416}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=17558416}, day = {10}, doi = {10.1038/nsmb1250}, issn = {1545-9993}, journal = {Nature structural \& molecular biology}, keywords = {hnrnp, intron, mirna, pri-mirna, splicing}, month = {July}, number = {7}, pages = {591--596}, posted-at = {2007-09-15 21:14:21}, priority = {3}, publisher = {Nature Publishing Group}, title = {The multifunctional RNA-binding protein hnRNP A1 is required for processing of miR-18a.}, url = {http://dx.doi.org/10.1038/nsmb1250}, volume = {14}, year = {2007} }

TY - JOUR ID - citeulike:1440426 L3 - citeulike-article-id:1440426 N2 - hnRNP A1 is an RNA-binding protein involved in various aspects of RNA processing. Use of an in vivo cross-linking and immunoprecipitation protocol to find hnRNP A1 RNA targets resulted in the identification of a microRNA (miRNA) precursor, pre-miR-18a. This microRNA is expressed as part of a cluster of intronic RNAs, including miR-17, miR-18a, miR-19a, miR-20a, miR-19b-1 and miR-92, and potentially acts as an oncogene. Here we show that hnRNP A1 binds specifically to the primary RNA sequence pri-miR-18a before Drosha processing. HeLa cells depleted of hnRNP A1 have reduced in vitro processing activity with pri-miR-18a and also show reduced abundances of endogenous pre-miR-18a. Furthermore, we show that hnRNP A1 is required for miR-18a-mediated repression of a target reporter in vivo. These results underscore a previously uncharacterized role for general RNA-binding proteins as auxiliary factors that facilitate the processing of specific miRNAs. IS - 7 JF - Nature structural & molecular biology SN - 1545-9993 EP - 596 TI - The multifunctional RNA-binding protein hnRNP A1 is required for processing of miR-18a. PB - Nature Publishing Group VL - 14 SP - 591 KW - hnrnp KW - intron KW - mirna KW - pri-mirna KW - splicing AU - Guil, Sonia AU - Cáceres, Javier PY - 2007/07/10/ UR - http://dx.doi.org/10.1038/nsmb1250 ER -