Z-DNA-binding proteins can act as potent effectors of gene expression invivo Export

@article{citeulike:6087269, abstract = {10.1073/pnas.262672699 The role of Z-DNA-binding proteins is explored in yeast. A conformation-specific yeast one-hybrid system is made in which formation of Z-DNA is studied near a minimal promoter site where it can be stabilized by negative supercoiling in addition to protein binding. Experiments were carried out with a Z-DNA-binding protein domain from the editing enzyme, double-stranded RNA adenosine deaminase 1. In the one-hybrid system, the reporter gene is activated when a Z-DNA-specific binding domain is fused with an activation domain and expressed . Significantly, it was found that even in the absence of the activation domain there is substantial transcription of the reporter gene if the Z-DNA-binding protein is expressed in the cell. This result suggests that Z-DNA formation in the promoter region induced or stabilized by a Z-DNA-binding protein can act as a -element in gene regulation. Related results have been found recently when the human chromatin-remodeling system converts a segment of DNA in the promoter region of the human colony-stimulating factor 1 gene into the left-handed Z-conformation.}, author = {Oh, Doo-Byoung and Kim, Yang-Gyun and Rich, Alexander}, citeulike-article-id = {6087269}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.262672699}, citeulike-linkout-1 = {http://www.pnas.org/content/99/26/16666.abstract}, citeulike-linkout-2 = {http://www.pnas.org/content/99/26/16666.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/12486233}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=12486233}, day = {24}, doi = {10.1073/pnas.262672699}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, keywords = {z-dna}, month = {December}, number = {26}, pages = {16666--16671}, posted-at = {2009-11-09 08:11:23}, priority = {2}, title = {Z-DNA-binding proteins can act as potent effectors of gene expression invivo}, url = {http://dx.doi.org/10.1073/pnas.262672699}, volume = {99}, year = {2002} }

TY - JOUR ID - citeulike:6087269 L3 - citeulike-article-id:6087269 N2 - 10.1073/pnas.262672699 The role of Z-DNA-binding proteins is explored in yeast. A conformation-specific yeast one-hybrid system is made in which formation of Z-DNA is studied near a minimal promoter site where it can be stabilized by negative supercoiling in addition to protein binding. Experiments were carried out with a Z-DNA-binding protein domain from the editing enzyme, double-stranded RNA adenosine deaminase 1. In the one-hybrid system, the reporter gene is activated when a Z-DNA-specific binding domain is fused with an activation domain and expressed . Significantly, it was found that even in the absence of the activation domain there is substantial transcription of the reporter gene if the Z-DNA-binding protein is expressed in the cell. This result suggests that Z-DNA formation in the promoter region induced or stabilized by a Z-DNA-binding protein can act as a -element in gene regulation. Related results have been found recently when the human chromatin-remodeling system converts a segment of DNA in the promoter region of the human colony-stimulating factor 1 gene into the left-handed Z-conformation. IS - 26 JF - Proceedings of the National Academy of Sciences of the United States of America EP - 16671 TI - Z-DNA-binding proteins can act as potent effectors of gene expression invivo VL - 99 SP - 16666 KW - z-dna AU - Oh, Doo-Byoung AU - Kim, Yang-Gyun AU - Rich, Alexander PY - 2002/12/24/ UR - http://dx.doi.org/10.1073/pnas.262672699 ER -