Highly Selective DNA-Based Sensor for Lead(II) and Mercury(II) Ions. Export

@article{citeulike:4832885, abstract = {We have developed a technique for the highly selective and sensitive detection of Pb(2+) and Hg(2+) using a thrombin-binding aptamer (TBA) probe labeled with the donor carboxyfluorescein (FAM) and the quencher 4-([4-(dimethylamino)phenyl]azo)benzoic acid (DABCYL) at its 5' and 3' termini, respectively. The TBA has a random coil structure that changes into a G-quartet structure and a hairpin-like structure upon binding Pb(2+) and Hg(2+) ions, respectively. As a result, the fluorescence decreases through fluorescence resonance energy transfer (FRET) between the fluorophore and quencher. These changes in fluorescence intensity allow the selective detection of Pb(2+) and Hg(2+) ions at concentrations as low as 300 pM and 5.0 nM using this TBA probe in the presence of phytic acid and a random DNA/NaCN mixture, respectively. The linear correlation existed between the fluorescence intensity and the concentration of Pb(2+) and Hg(2+) over the range of 0.5-30 nM (R(2) = 0.98) and 10-200 nM (R(2) = 0.98), respectively. To the best of our knowledge, this is the first example of a single DNA-based sensor that allows the detection of both Hg(2+) and Pb(2+) ions. This simple and cost-effective probe was also applied to separately determine Pb(2+) in soil samples and spiked Hg(2+) in pond samples.}, author = {Liu, Chi-Wei W. and Huang, Chih-Ching C. and Chang, Huan-Tsung T.}, citeulike-article-id = {4832885}, citeulike-linkout-0 = {http://dx.doi.org/10.1021/ac8022185}, citeulike-linkout-1 = {http://pubs.acs.org/doi/abs/10.1021/ac8022185}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19219985}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19219985}, day = {16}, doi = {10.1021/ac8022185}, issn = {1520-6882}, journal = {Analytical chemistry}, keywords = {lead, lead\_testing, mercury, water\_chemistry, water\_lead}, month = {February}, posted-at = {2009-10-22 20:13:58}, priority = {2}, title = {Highly Selective DNA-Based Sensor for Lead(II) and Mercury(II) Ions.}, url = {http://dx.doi.org/10.1021/ac8022185}, year = {2009} }

TY - JOUR ID - citeulike:4832885 L3 - citeulike-article-id:4832885 N2 - We have developed a technique for the highly selective and sensitive detection of Pb(2+) and Hg(2+) using a thrombin-binding aptamer (TBA) probe labeled with the donor carboxyfluorescein (FAM) and the quencher 4-([4-(dimethylamino)phenyl]azo)benzoic acid (DABCYL) at its 5' and 3' termini, respectively. The TBA has a random coil structure that changes into a G-quartet structure and a hairpin-like structure upon binding Pb(2+) and Hg(2+) ions, respectively. As a result, the fluorescence decreases through fluorescence resonance energy transfer (FRET) between the fluorophore and quencher. These changes in fluorescence intensity allow the selective detection of Pb(2+) and Hg(2+) ions at concentrations as low as 300 pM and 5.0 nM using this TBA probe in the presence of phytic acid and a random DNA/NaCN mixture, respectively. The linear correlation existed between the fluorescence intensity and the concentration of Pb(2+) and Hg(2+) over the range of 0.5-30 nM (R(2) = 0.98) and 10-200 nM (R(2) = 0.98), respectively. To the best of our knowledge, this is the first example of a single DNA-based sensor that allows the detection of both Hg(2+) and Pb(2+) ions. This simple and cost-effective probe was also applied to separately determine Pb(2+) in soil samples and spiked Hg(2+) in pond samples. JF - Analytical chemistry SN - 1520-6882 TI - Highly Selective DNA-Based Sensor for Lead(II) and Mercury(II) Ions. KW - lead KW - lead_testing KW - mercury KW - water_chemistry KW - water_lead AU - Liu, Chi-Wei AU - Huang, Chih-Ching AU - Chang, Huan-Tsung PY - 2009/02/16/ UR - http://dx.doi.org/10.1021/ac8022185 ER -