Generation of monoclonal antibody that distinguishes PrP(Sc) from PrP(C) and neutralizes prion infectivity. Export

@article{citeulike:5828268, abstract = {To establish PrP(Sc)-specific mAbs, we immunized Prnp(-/-) mice with PrP(Sc) purified from prion-infected mice. Using this approach, we obtained mAb 6H10, which reacted with PrP(Sc) treated with proteinase K, but not with PrP(Sc) pretreated with more than 3 M GdnHCl. In contrast, reactivity of pan-PrP mAbs increased with increasing concentrations of GdnHCl used for pretreatment of PrP(Sc). In histoblot analysis, mAb 6H10 showed a positive reaction on a non-denatured histoblot but reactivity was lower when the histoblot was pretreated by autoclaving. Epitope analysis suggested that the extreme C-terminus of PrP is likely to be part of the epitope for mAb 6H10. MAb 6H10 immunoprecipitated PrP(Sc) from brains of mice, sheep, and cattle infected with prions. Furthermore, pretreatment of purified PrP(Sc) with mAb 6H10 reduced the infectious titer more than 1 log. Taken together, these results suggest that mAb 6H10 recognizes a conformational epitope on PrP(Sc) that is related to prion infectivity.}, author = {Horiuchi, Motohiro and Karino, Ayako and Furuoka, Hidefumi and Ishiguro, Naotaka and Kimura, Kumiko and Shinagawa, Morikazu}, citeulike-article-id = {5828268}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.virol.2009.08.025}, citeulike-linkout-1 = {http://linkinghub.elsevier.com/retrieve/pii/S0042682209005182}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19766283}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19766283}, day = {17}, doi = {10.1016/j.virol.2009.08.025}, issn = {1096-0341}, journal = {Virology}, keywords = {antibody, prion, treatment}, month = {September}, posted-at = {2009-09-23 15:34:43}, priority = {4}, title = {Generation of monoclonal antibody that distinguishes PrP(Sc) from PrP(C) and neutralizes prion infectivity.}, url = {http://dx.doi.org/10.1016/j.virol.2009.08.025}, year = {2009} }

TY - JOUR ID - citeulike:5828268 L3 - citeulike-article-id:5828268 N2 - To establish PrP(Sc)-specific mAbs, we immunized Prnp(-/-) mice with PrP(Sc) purified from prion-infected mice. Using this approach, we obtained mAb 6H10, which reacted with PrP(Sc) treated with proteinase K, but not with PrP(Sc) pretreated with more than 3 M GdnHCl. In contrast, reactivity of pan-PrP mAbs increased with increasing concentrations of GdnHCl used for pretreatment of PrP(Sc). In histoblot analysis, mAb 6H10 showed a positive reaction on a non-denatured histoblot but reactivity was lower when the histoblot was pretreated by autoclaving. Epitope analysis suggested that the extreme C-terminus of PrP is likely to be part of the epitope for mAb 6H10. MAb 6H10 immunoprecipitated PrP(Sc) from brains of mice, sheep, and cattle infected with prions. Furthermore, pretreatment of purified PrP(Sc) with mAb 6H10 reduced the infectious titer more than 1 log. Taken together, these results suggest that mAb 6H10 recognizes a conformational epitope on PrP(Sc) that is related to prion infectivity. JF - Virology SN - 1096-0341 TI - Generation of monoclonal antibody that distinguishes PrP(Sc) from PrP(C) and neutralizes prion infectivity. KW - antibody KW - prion KW - treatment AU - Horiuchi, Motohiro AU - Karino, Ayako AU - Furuoka, Hidefumi AU - Ishiguro, Naotaka AU - Kimura, Kumiko AU - Shinagawa, Morikazu PY - 2009/09/17/ UR - http://dx.doi.org/10.1016/j.virol.2009.08.025 ER -