Chromatin analysis of occluded genes Export

@article{citeulike:4789786, abstract = {We recently described two opposing states of transcriptional competency. One is termed competent' whereby a gene is capable of responding to trans-acting transcription factors of the cell, such that it is active if appropriate transcriptional activators are present, though it can also be silent if activators are absent or repressors are present. The other is termed occluded' whereby a gene is silenced by cis-acting, chromatin-based mechanisms in a manner that blocks it from responding to trans-acting factors, such that it is silent even when activators are present in the cellular milieu. We proposed that gene occlusion is a mechanism by which differentiated cells stably maintain their phenotypic identities. Here, we describe chromatin analysis of occluded genes. We found that DNA methylation plays a causal role in maintaining occlusion for a subset of occluded genes. We further examined a variety of other chromatin marks typically associated with transcriptional silencing, including histone variants, covalent histone modifications and chromatin-associated proteins. Surprisingly, we found that although many of these marks are robustly linked to silent genes (which include both occluded genes and genes that are competent but silent), none is linked specifically to occluded genes. Although the observation does not rule out a possible causal role of these chromatin marks in occlusion, it does suggest that these marks might be secondary effect rather than primary cause of the silent state in many genes. 10.1093/hmg/ddp188}, author = {Lee, Jae H. and Gaetz, Jedidiah and Bugarija, Branimir and Fernandes, Croydon J. and Snyder, Gregory E. and Bush, Eliot C. and Lahn, Bruce T.}, citeulike-article-id = {4789786}, citeulike-linkout-0 = {http://dx.doi.org/10.1093/hmg/ddp188}, citeulike-linkout-1 = {http://hmg.oxfordjournals.org/cgi/content/abstract/18/14/2567}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19380460}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19380460}, day = {15}, doi = {10.1093/hmg/ddp188}, journal = {Hum. Mol. Genet.}, keywords = {chromatin, cis-acting, methylation, occlusion}, month = {July}, number = {14}, pages = {2567--2574}, posted-at = {2009-11-05 14:55:35}, priority = {2}, title = {Chromatin analysis of occluded genes}, url = {http://dx.doi.org/10.1093/hmg/ddp188}, volume = {18}, year = {2009} }

TY - JOUR ID - citeulike:4789786 L3 - citeulike-article-id:4789786 N2 - We recently described two opposing states of transcriptional competency. One is termed competent' whereby a gene is capable of responding to trans-acting transcription factors of the cell, such that it is active if appropriate transcriptional activators are present, though it can also be silent if activators are absent or repressors are present. The other is termed occluded' whereby a gene is silenced by cis-acting, chromatin-based mechanisms in a manner that blocks it from responding to trans-acting factors, such that it is silent even when activators are present in the cellular milieu. We proposed that gene occlusion is a mechanism by which differentiated cells stably maintain their phenotypic identities. Here, we describe chromatin analysis of occluded genes. We found that DNA methylation plays a causal role in maintaining occlusion for a subset of occluded genes. We further examined a variety of other chromatin marks typically associated with transcriptional silencing, including histone variants, covalent histone modifications and chromatin-associated proteins. Surprisingly, we found that although many of these marks are robustly linked to silent genes (which include both occluded genes and genes that are competent but silent), none is linked specifically to occluded genes. Although the observation does not rule out a possible causal role of these chromatin marks in occlusion, it does suggest that these marks might be secondary effect rather than primary cause of the silent state in many genes. 10.1093/hmg/ddp188 IS - 14 JF - Hum. Mol. Genet. EP - 2574 TI - Chromatin analysis of occluded genes VL - 18 SP - 2567 KW - chromatin KW - cis-acting KW - methylation KW - occlusion AU - Lee, Jae AU - Gaetz, Jedidiah AU - Bugarija, Branimir AU - Fernandes, Croydon AU - Snyder, Gregory AU - Bush, Eliot AU - Lahn, Bruce PY - 2009/07/15/ UR - http://dx.doi.org/10.1093/hmg/ddp188 ER -