The terminal DNA structure of mammalian chromosomes. Export

@article{citeulike:1168071, abstract = {In virtually all eukaryotic organisms, telomeric DNA is composed of a variable number of short direct repeats. While the primary sequence of telomeric repeats has been determined for a great variety of species, the actual physical DNA structure at the ends of a bona fide metazoan chromosome with a centromere is unknown. It is shown here that an overhang of the strand forming the 3' ends of the chromosomes, the G-rich strand, is found at mammalian chromosome ends. Moreover, on at least some telomeres, the overhangs are > or = 45 bases long. Such surprisingly long overhangs were present on chromosomes derived from fully transformed tissue culture cells and normal G0-arrested peripheral leukocytes. Thus, irrespective of whether the cells were actively dividing or arrested, a very similar terminal DNA arrangement was found. These data suggest that the ends of mammalian and possibly all vertebrate chromosomes consist of an overhang of the G-rich strand and that these overhangs may be considerably larger than previously anticipated.}, address = {Dept. de Microbiologie et Infectiologie, Facult\'{e} de M\'{e}decine, Universit\'{e} de Sherbrooke, QC, Canada.}, author = {McElligott, R. and Wellinger, R. J.}, citeulike-article-id = {1168071}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/9218811}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=9218811}, day = {16}, issn = {0261-4189}, journal = {EMBO J}, keywords = {overhang}, month = {June}, number = {12}, pages = {3705--3714}, posted-at = {2007-03-16 23:35:49}, priority = {4}, title = {The terminal DNA structure of mammalian chromosomes.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/9218811}, volume = {16}, year = {1997} }

TY - JOUR ID - citeulike:1168071 L3 - citeulike-article-id:1168071 N2 - In virtually all eukaryotic organisms, telomeric DNA is composed of a variable number of short direct repeats. While the primary sequence of telomeric repeats has been determined for a great variety of species, the actual physical DNA structure at the ends of a bona fide metazoan chromosome with a centromere is unknown. It is shown here that an overhang of the strand forming the 3' ends of the chromosomes, the G-rich strand, is found at mammalian chromosome ends. Moreover, on at least some telomeres, the overhangs are > or = 45 bases long. Such surprisingly long overhangs were present on chromosomes derived from fully transformed tissue culture cells and normal G0-arrested peripheral leukocytes. Thus, irrespective of whether the cells were actively dividing or arrested, a very similar terminal DNA arrangement was found. These data suggest that the ends of mammalian and possibly all vertebrate chromosomes consist of an overhang of the G-rich strand and that these overhangs may be considerably larger than previously anticipated. IS - 12 JF - EMBO J SN - 0261-4189 AD - Dept. de Microbiologie et Infectiologie, Faculté de Médecine, Université de Sherbrooke, QC, Canada. EP - 3714 TI - The terminal DNA structure of mammalian chromosomes. VL - 16 SP - 3705 KW - overhang AU - McElligott, R AU - Wellinger, RJ PY - 1997/06/16/ UR - http://view.ncbi.nlm.nih.gov/pubmed/9218811 ER -