Strand-specific postreplicative processing of mammalian telomeres. Export

@article{citeulike:1168117, abstract = {Telomeres are specialized nucleoprotein structures that stabilize the ends of linear eukaryotic chromosomes. In mammalian cells, abrogation of telomeric repeat binding factor TRF2 or DNA-dependent protein kinase (DNA-PK) activity causes end-to-end chromosomal fusion, thus establishing an essential role for these proteins in telomere function. Here we show that TRF2-mediated end-capping occurs after telomere replication. The postreplicative requirement for TRF2 and DNA-PKcs, the catalytic subunit of DNA-PK, is confined to only half of the telomeres, namely, those that were produced by leading-strand DNA synthesis. These results demonstrate a crucial difference in postreplicative processing of telomeres that is linked to their mode of replication.}, address = {Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM 87544, USA.}, author = {Bailey, S. M. and Cornforth, M. N. and Kurimasa, A. and Chen, D. J. and Goodwin, E. H.}, citeulike-article-id = {1168117}, citeulike-linkout-0 = {http://dx.doi.org/10.1126/science.1062560}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/11577237}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=11577237}, day = {28}, doi = {10.1126/science.1062560}, issn = {0036-8075}, journal = {Science}, keywords = {capping, fusion, trf2}, month = {September}, number = {5539}, pages = {2462--2465}, posted-at = {2007-03-17 01:04:41}, priority = {2}, title = {Strand-specific postreplicative processing of mammalian telomeres.}, url = {http://dx.doi.org/10.1126/science.1062560}, volume = {293}, year = {2001} }

TY - JOUR ID - citeulike:1168117 L3 - citeulike-article-id:1168117 N2 - Telomeres are specialized nucleoprotein structures that stabilize the ends of linear eukaryotic chromosomes. In mammalian cells, abrogation of telomeric repeat binding factor TRF2 or DNA-dependent protein kinase (DNA-PK) activity causes end-to-end chromosomal fusion, thus establishing an essential role for these proteins in telomere function. Here we show that TRF2-mediated end-capping occurs after telomere replication. The postreplicative requirement for TRF2 and DNA-PKcs, the catalytic subunit of DNA-PK, is confined to only half of the telomeres, namely, those that were produced by leading-strand DNA synthesis. These results demonstrate a crucial difference in postreplicative processing of telomeres that is linked to their mode of replication. IS - 5539 JF - Science SN - 0036-8075 AD - Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM 87544, USA. EP - 2465 TI - Strand-specific postreplicative processing of mammalian telomeres. VL - 293 SP - 2462 KW - capping KW - fusion KW - trf2 AU - Bailey, SM AU - Cornforth, MN AU - Kurimasa, A AU - Chen, DJ AU - Goodwin, EH PY - 2001/09/28/ UR - http://dx.doi.org/10.1126/science.1062560 ER -