Long G tails at both ends of human chromosomes suggest a C strand degradation mechanism for telomere shortening. Export

@article{citeulike:1186328, abstract = {The chromosomes of lower eukaryotes have short telomeric 3' extensions. Using a primer-extension/nick-translation technique and nondenaturing hybridization, we find long 3' G-rich tails at human chromosome ends in mortal primary fibroblasts, umbilical vein endothelial cells, and leukocytes, as well as in immortalized fibroblasts. For all cells tested, >80\% of the telomeres have long G-rich overhangs, averaging 130-210 bases in length, in disagreement with the conventional model for incomplete lagging-strand replication, which predicts overhangs on 50\% of the chromosome ends. The observed G tails must exist during most of the cell cycle and probably result from degradation of both chromosome ends. The average lengths of the G tails are quantitatively consistent with the observed rates of human chromosome shortening.}, address = {Biophysics Research Division, University of Michigan, Ann Arbor 48109-1055, USA.}, author = {Makarov, V. L. and Hirose, Y. and Langmore, J. P.}, citeulike-article-id = {1186328}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/9054505}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=9054505}, day = {7}, issn = {0092-8674}, journal = {Cell}, keywords = {capping, overhang}, month = {March}, number = {5}, pages = {657--666}, posted-at = {2007-03-25 19:43:50}, priority = {2}, title = {Long G tails at both ends of human chromosomes suggest a C strand degradation mechanism for telomere shortening.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/9054505}, volume = {88}, year = {1997} }

TY - JOUR ID - citeulike:1186328 L3 - citeulike-article-id:1186328 N2 - The chromosomes of lower eukaryotes have short telomeric 3' extensions. Using a primer-extension/nick-translation technique and nondenaturing hybridization, we find long 3' G-rich tails at human chromosome ends in mortal primary fibroblasts, umbilical vein endothelial cells, and leukocytes, as well as in immortalized fibroblasts. For all cells tested, >80% of the telomeres have long G-rich overhangs, averaging 130-210 bases in length, in disagreement with the conventional model for incomplete lagging-strand replication, which predicts overhangs on 50% of the chromosome ends. The observed G tails must exist during most of the cell cycle and probably result from degradation of both chromosome ends. The average lengths of the G tails are quantitatively consistent with the observed rates of human chromosome shortening. IS - 5 JF - Cell SN - 0092-8674 AD - Biophysics Research Division, University of Michigan, Ann Arbor 48109-1055, USA. EP - 666 TI - Long G tails at both ends of human chromosomes suggest a C strand degradation mechanism for telomere shortening. VL - 88 SP - 657 KW - capping KW - overhang AU - Makarov, VL AU - Hirose, Y AU - Langmore, JP PY - 1997/03/07/ UR - http://view.ncbi.nlm.nih.gov/pubmed/9054505 ER -