Protein composition of catalytically active human telomerase from immortal cells. Export

@article{citeulike:1203489, abstract = {Telomerase is a ribonucleoprotein enzyme complex that adds 5'-TTAGGG-3' repeats onto the ends of human chromosomes, providing a telomere maintenance mechanism for approximately 90\% of human cancers. We have purified human telomerase approximately 10(8)-fold, with the final elution dependent on the enzyme's ability to catalyze nucleotide addition onto a DNA oligonucleotide of telomeric sequence, thereby providing specificity for catalytically active telomerase. Mass spectrometric sequencing of the protein components and molecular size determination indicated an enzyme composition of two molecules each of telomerase reverse transcriptase, telomerase RNA, and dyskerin.}, address = {Cancer Research Unit, Children's Medical Research Institute, 214 Hawkesbury Road, Westmead NSW 2145, Australia.}, author = {Cohen, S. B. and Graham, M. E. and Lovrecz, G. O. and Bache, N. and Robinson, P. J. and Reddel, R. R.}, citeulike-article-id = {1203489}, citeulike-linkout-0 = {http://dx.doi.org/10.1126/science.1138596}, citeulike-linkout-1 = {http://www.sciencemag.org/cgi/content/abstract/315/5820/1850}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/17395830}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=17395830}, day = {30}, doi = {10.1126/science.1138596}, issn = {1095-9203}, journal = {Science}, keywords = {landmark, telomerasesubunits}, month = {March}, number = {5820}, pages = {1850--1853}, posted-at = {2007-04-03 01:25:05}, priority = {2}, title = {Protein composition of catalytically active human telomerase from immortal cells.}, url = {http://dx.doi.org/10.1126/science.1138596}, volume = {315}, year = {2007} }

TY - JOUR ID - citeulike:1203489 L3 - citeulike-article-id:1203489 N2 - Telomerase is a ribonucleoprotein enzyme complex that adds 5'-TTAGGG-3' repeats onto the ends of human chromosomes, providing a telomere maintenance mechanism for approximately 90% of human cancers. We have purified human telomerase approximately 10(8)-fold, with the final elution dependent on the enzyme's ability to catalyze nucleotide addition onto a DNA oligonucleotide of telomeric sequence, thereby providing specificity for catalytically active telomerase. Mass spectrometric sequencing of the protein components and molecular size determination indicated an enzyme composition of two molecules each of telomerase reverse transcriptase, telomerase RNA, and dyskerin. IS - 5820 JF - Science SN - 1095-9203 AD - Cancer Research Unit, Children's Medical Research Institute, 214 Hawkesbury Road, Westmead NSW 2145, Australia. EP - 1853 TI - Protein composition of catalytically active human telomerase from immortal cells. VL - 315 SP - 1850 KW - landmark KW - telomerasesubunits AU - Cohen, SB AU - Graham, ME AU - Lovrecz, GO AU - Bache, N AU - Robinson, PJ AU - Reddel, RR PY - 2007/03/30/ UR - http://dx.doi.org/10.1126/science.1138596 ER -