Telomere shortening and tumor formation by mouse cells lacking telomerase RNA. Export

@article{citeulike:1264066, abstract = {To examine the role of telomerase in normal and neoplastic growth, the telomerase RNA component (mTR) was deleted from the mouse germline. mTR-/- mice lacked detectable telomerase activity yet were viable for the six generations analyzed. Telomerase-deficient cells could be immortalized in culture, transformed by viral oncogenes, and generated tumors in nude mice following transformation. Telomeres were shown to shorten at a rate of 4.8+/-2.4 kb per mTR-/- generation. Cells from the fourth mTR-/- generation onward possessed chromosome ends lacking detectable telomere repeats, aneuploidy, and chromosomal abnormalities, including end-to-end fusions. These results indicate that telomerase is essential for telomere length maintenance but is not required for establishment of cell lines, oncogenic transformation, or tumor formation in mice.}, address = {Cold Spring Harbor Laboratory, New York 11724, USA.}, author = {Blasco, M. A. and Lee, H. W. and Hande, M. P. and Samper, E. and Lansdorp, P. M. and DePinho, R. A. and Greider, C. W.}, citeulike-article-id = {1264066}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/9335332}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=9335332}, day = {3}, issn = {0092-8674}, journal = {Cell}, keywords = {cancer, trap}, month = {October}, number = {1}, pages = {25--34}, posted-at = {2007-04-29 04:30:34}, priority = {2}, title = {Telomere shortening and tumor formation by mouse cells lacking telomerase RNA.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/9335332}, volume = {91}, year = {1997} }

TY - JOUR ID - citeulike:1264066 L3 - citeulike-article-id:1264066 N2 - To examine the role of telomerase in normal and neoplastic growth, the telomerase RNA component (mTR) was deleted from the mouse germline. mTR-/- mice lacked detectable telomerase activity yet were viable for the six generations analyzed. Telomerase-deficient cells could be immortalized in culture, transformed by viral oncogenes, and generated tumors in nude mice following transformation. Telomeres were shown to shorten at a rate of 4.8+/-2.4 kb per mTR-/- generation. Cells from the fourth mTR-/- generation onward possessed chromosome ends lacking detectable telomere repeats, aneuploidy, and chromosomal abnormalities, including end-to-end fusions. These results indicate that telomerase is essential for telomere length maintenance but is not required for establishment of cell lines, oncogenic transformation, or tumor formation in mice. IS - 1 JF - Cell SN - 0092-8674 AD - Cold Spring Harbor Laboratory, New York 11724, USA. EP - 34 TI - Telomere shortening and tumor formation by mouse cells lacking telomerase RNA. VL - 91 SP - 25 KW - cancer KW - trap AU - Blasco, MA AU - Lee, HW AU - Hande, MP AU - Samper, E AU - Lansdorp, PM AU - DePinho, RA AU - Greider, CW PY - 1997/10/03/ UR - http://view.ncbi.nlm.nih.gov/pubmed/9335332 ER -