Telomere Maintenance in Telomerase-Deficient Mouse Embryonic Stem Cells: Characterization of an Amplified Telomeric DNA Export

@article{citeulike:1278785, abstract = {Telomere dynamics, chromosomal instability, and cellular viability were studied in serial passages of mouse embryonic stem (ES) cells in which the telomerase RNA (mTER) gene was deleted. These cells lack detectable telomerase activity, and their growth rate was reduced after more than 300 divisions and almost zero after 450 cell divisions. After this growth crisis, survivor cells with a rapid growth rate did emerge. Such survivors were found to maintain functional telomeres in a telomerase-independent fashion. Although telomerase-independent telomere maintenance has been reported for some immortalized mammalian cells, its molecular mechanism has not been elucidated. Characterization of the telomeric structures in one of the survivor mTER[-]/[-] cell lines showed amplification of the same tandem arrays of telomeric and nontelomeric sequences at most of the chromosome ends. This evidence implicates cis/trans amplification as one mechanism for the telomerase-independent maintenance of telomeres in mammalian cells. 10.1128/MCB.20.11.4115-4127.2000}, author = {Niida, Hiroyuki and Shinkai, Yoichi and Hande, Prakash M. and Matsumoto, Takehisa and Takehara, Shoko and Tachibana, Makoto and Oshimura, Mitsuo and Lansdorp, Peter M. and Furuichi, Yasuhiro}, citeulike-article-id = {1278785}, citeulike-linkout-0 = {http://mcb.asm.org/cgi/content/abstract/20/11/4115}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/10805753}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=10805753}, day = {1}, journal = {Mol. Cell. Biol.}, keywords = {alt}, month = {June}, number = {11}, pages = {4115--4127}, posted-at = {2007-05-05 09:16:02}, priority = {2}, title = {Telomere Maintenance in Telomerase-Deficient Mouse Embryonic Stem Cells: Characterization of an Amplified Telomeric DNA}, url = {http://mcb.asm.org/cgi/content/abstract/20/11/4115}, volume = {20}, year = {2000} }

TY - JOUR ID - citeulike:1278785 L3 - citeulike-article-id:1278785 N2 - Telomere dynamics, chromosomal instability, and cellular viability were studied in serial passages of mouse embryonic stem (ES) cells in which the telomerase RNA (mTER) gene was deleted. These cells lack detectable telomerase activity, and their growth rate was reduced after more than 300 divisions and almost zero after 450 cell divisions. After this growth crisis, survivor cells with a rapid growth rate did emerge. Such survivors were found to maintain functional telomeres in a telomerase-independent fashion. Although telomerase-independent telomere maintenance has been reported for some immortalized mammalian cells, its molecular mechanism has not been elucidated. Characterization of the telomeric structures in one of the survivor mTER[-]/[-] cell lines showed amplification of the same tandem arrays of telomeric and nontelomeric sequences at most of the chromosome ends. This evidence implicates cis/trans amplification as one mechanism for the telomerase-independent maintenance of telomeres in mammalian cells. 10.1128/MCB.20.11.4115-4127.2000 IS - 11 JF - Mol. Cell. Biol. EP - 4127 TI - Telomere Maintenance in Telomerase-Deficient Mouse Embryonic Stem Cells: Characterization of an Amplified Telomeric DNA VL - 20 SP - 4115 KW - alt AU - Niida, Hiroyuki AU - Shinkai, Yoichi AU - Hande, Prakash AU - Matsumoto, Takehisa AU - Takehara, Shoko AU - Tachibana, Makoto AU - Oshimura, Mitsuo AU - Lansdorp, Peter AU - Furuichi, Yasuhiro PY - 2000/06/01/ UR - http://mcb.asm.org/cgi/content/abstract/20/11/4115 ER -