Cholesterol Metabolism: the Main Pathway Acting Downstream of Cytochrome P450 Oxidoreductase in Skeletal Development of the Limb Export

@article{citeulike:4729980, abstract = {Cytochrome P450 oxidoreductase (POR) is the obligate electron donor for all microsomal cytochrome P450 enzymes, which catalyze the metabolism of a wide spectrum of xenobiotic and endobiotic compounds. Point mutations in POR have been found recently in patients with Antley-Bixler-like syndrome, which includes limb skeletal defects. In order to study P450 function during limb and skeletal development, we deleted POR specifically in mouse limb bud mesenchyme. Forelimbs and hind limbs in conditional knockout (CKO) mice were short with thin skeletal elements and fused joints. POR deletion occurred earlier in forelimbs than in hind limbs, leading additionally to soft tissue syndactyly and loss of wrist elements and phalanges due to changes in growth, cell death, and skeletal segmentation. Transcriptional analysis of E12.5 mouse forelimb buds demonstrated the expression of P450s involved in retinoic acid, cholesterol, and arachidonic acid metabolism. Biochemical analysis of CKO limbs confirmed retinoic acid excess. In CKO limbs, expression of genes throughout the whole cholesterol biosynthetic pathway was upregulated, and cholesterol deficiency can explain most aspects of the phenotype. Thus, cellular POR-dependent cholesterol synthesis is essential during limb and skeletal development. Modulation of P450 activity could contribute to susceptibility of the embryo and developing organs to teratogenesis. 10.1128/MCB.01638-08}, author = {Schmidt, Katy and Hughes, Catherine and Chudek, J. A. and Goodyear, Simon R. and Aspden, Richard M. and Talbot, Richard and Gundersen, Thomas E. and Blomhoff, Rune and Henderson, Colin and Wolf, Roland C. and Tickle, Cheryll}, citeulike-article-id = {4729980}, citeulike-linkout-0 = {http://dx.doi.org/10.1128/MCB.01638-08}, citeulike-linkout-1 = {http://mcb.asm.org/cgi/content/abstract/29/10/2716}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19273610}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19273610}, day = {15}, doi = {10.1128/MCB.01638-08}, journal = {Mol. Cell. Biol.}, keywords = {genetics, microct, mouse}, month = {May}, number = {10}, pages = {2716--2729}, posted-at = {2009-06-03 08:13:17}, priority = {2}, title = {Cholesterol Metabolism: the Main Pathway Acting Downstream of Cytochrome P450 Oxidoreductase in Skeletal Development of the Limb}, url = {http://dx.doi.org/10.1128/MCB.01638-08}, volume = {29}, year = {2009} }

TY - JOUR ID - citeulike:4729980 L3 - citeulike-article-id:4729980 N2 - Cytochrome P450 oxidoreductase (POR) is the obligate electron donor for all microsomal cytochrome P450 enzymes, which catalyze the metabolism of a wide spectrum of xenobiotic and endobiotic compounds. Point mutations in POR have been found recently in patients with Antley-Bixler-like syndrome, which includes limb skeletal defects. In order to study P450 function during limb and skeletal development, we deleted POR specifically in mouse limb bud mesenchyme. Forelimbs and hind limbs in conditional knockout (CKO) mice were short with thin skeletal elements and fused joints. POR deletion occurred earlier in forelimbs than in hind limbs, leading additionally to soft tissue syndactyly and loss of wrist elements and phalanges due to changes in growth, cell death, and skeletal segmentation. Transcriptional analysis of E12.5 mouse forelimb buds demonstrated the expression of P450s involved in retinoic acid, cholesterol, and arachidonic acid metabolism. Biochemical analysis of CKO limbs confirmed retinoic acid excess. In CKO limbs, expression of genes throughout the whole cholesterol biosynthetic pathway was upregulated, and cholesterol deficiency can explain most aspects of the phenotype. Thus, cellular POR-dependent cholesterol synthesis is essential during limb and skeletal development. Modulation of P450 activity could contribute to susceptibility of the embryo and developing organs to teratogenesis. 10.1128/MCB.01638-08 IS - 10 JF - Mol. Cell. Biol. EP - 2729 TI - Cholesterol Metabolism: the Main Pathway Acting Downstream of Cytochrome P450 Oxidoreductase in Skeletal Development of the Limb VL - 29 SP - 2716 KW - genetics KW - microct KW - mouse AU - Schmidt, Katy AU - Hughes, Catherine AU - Chudek, JA AU - Goodyear, Simon AU - Aspden, Richard AU - Talbot, Richard AU - Gundersen, Thomas AU - Blomhoff, Rune AU - Henderson, Colin AU - Wolf, Roland AU - Tickle, Cheryll PY - 2009/05/15/ UR - http://dx.doi.org/10.1128/MCB.01638-08 ER -