Mixed and diverse metabolic and gene-expression regulation of the glycolytic and fermentative pathways in response to a HXK2 deletion in Saccharomyces cerevisiae Export

@article{citeulike:2098873, abstract = {Abstract In Saccharomyces cerevisiae the HXK2 gene, which encodes the glycolytic enzyme hexokinase II, is involved in the regulatory mechanism known as 'glucose repression'. Its deletion leads to fully respiratory growth at high glucose concentrations where the wild type ferments profusely. Here we describe that deletion of the HXK2 gene resulted in a 75\% reduction in fermentative capacity. Using regulation analysis we found that the fluxes through most glycolytic and fermentative enzymes were regulated cooperatively by changes in their capacities (Vmax) and by changes in the way they interacted with the rest of the metabolism. Glucose transport and phosphofructokinase were regulated purely at the metabolic level. The reduction of fermentative capacity in the mutant was accompanied by a remarkable resilience of the remaining capacity to nutrient starvation. After starvation, the fermentative capacity of the hxk2Delta mutant was similar to that of the wild type. Based on our results and previous reports, we suggest an inverse correlation between glucose repression and the resilience of fermentative capacity towards nutrient starvation. Only a limited number of glycolytic enzyme activities changed upon starvation of the hxk2Delta mutant and we discuss to what extent this could explain the stability of the fermentative capacity.}, author = {Rossell, Sergio and Lindenbergh, Alexander and van der Weijden, Coen C. and Kruckeberg, Arthur L. and van Eunen, Karen and Westerhoff, Hans V. and Bakker, Barbara M.}, citeulike-article-id = {2098873}, citeulike-linkout-0 = {http://www.blackwell-synergy.com/doi/abs/10.1111/j.1567-1364.2007.00282.x}, citeulike-linkout-1 = {http://dx.doi.org/10.1111/j.1567-1364.2007.00282.x}, citeulike-linkout-2 = {http://www3.interscience.wiley.com/cgi-bin/abstract/119423959/ABSTRACT}, doi = {10.1111/j.1567-1364.2007.00282.x}, journal = {FEMS Yeast Research}, keywords = {expression, fermentation, gene, glycolytic, metabolic, saccharomyces}, number = {0}, pages = {???}, posted-at = {2008-10-06 14:49:31}, priority = {2}, title = {Mixed and diverse metabolic and gene-expression regulation of the glycolytic and fermentative pathways in response to a HXK2 deletion in Saccharomyces cerevisiae}, url = {http://dx.doi.org/10.1111/j.1567-1364.2007.00282.x}, volume = {0}, year = {0} }

TY - JOUR ID - citeulike:2098873 L3 - citeulike-article-id:2098873 N2 - Abstract In Saccharomyces cerevisiae the HXK2 gene, which encodes the glycolytic enzyme hexokinase II, is involved in the regulatory mechanism known as ‘glucose repression’. Its deletion leads to fully respiratory growth at high glucose concentrations where the wild type ferments profusely. Here we describe that deletion of the HXK2 gene resulted in a 75% reduction in fermentative capacity. Using regulation analysis we found that the fluxes through most glycolytic and fermentative enzymes were regulated cooperatively by changes in their capacities (Vmax) and by changes in the way they interacted with the rest of the metabolism. Glucose transport and phosphofructokinase were regulated purely at the metabolic level. The reduction of fermentative capacity in the mutant was accompanied by a remarkable resilience of the remaining capacity to nutrient starvation. After starvation, the fermentative capacity of the hxk2Delta mutant was similar to that of the wild type. Based on our results and previous reports, we suggest an inverse correlation between glucose repression and the resilience of fermentative capacity towards nutrient starvation. Only a limited number of glycolytic enzyme activities changed upon starvation of the hxk2Delta mutant and we discuss to what extent this could explain the stability of the fermentative capacity. IS - 0 JF - FEMS Yeast Research EP - ??? TI - Mixed and diverse metabolic and gene-expression regulation of the glycolytic and fermentative pathways in response to a HXK2 deletion in Saccharomyces cerevisiae VL - 0 SP - ??? KW - expression KW - fermentation KW - gene KW - glycolytic KW - metabolic KW - saccharomyces AU - Rossell, Sergio AU - Lindenbergh, Alexander AU - van der Weijden, Coen AU - Kruckeberg, Arthur AU - van Eunen, Karen AU - Westerhoff, Hans AU - Bakker, Barbara PY - 2000/// UR - http://dx.doi.org/10.1111/j.1567-1364.2007.00282.x ER -