Quantitative 2H NMR at Natural Abundance Can Distinguish the Pathway Used for Glucose Fermentation by Lactic Acid Bacteria Export

@article{citeulike:2374184, abstract = {For any given metabolic pathway, isotope redistribution coefficients (aij) that characterize the specific derivation of each hydrogen atom can be defined. By using quantitative deuterium NMR, the redistribution of deuterium at natural abundance in lactic acid produced by the bacterial fermentation of glucose has been determined for each non-labile hydrogen atom of glucose or water and the hydrogen atoms of lactic acid. Distinct differences are observed in the lactic acid isolated from Lactococcus lactis and Leuconostoc mesenteroides that can be interpreted in terms of the different fermentative pathways used. Specifically, the affiliations observed between the H1, H3, and H4 positions of glucose with methyl and hydroxymethylene of lactic acid can give quantitative information on whether the glycolytic or the reductive pentose-phosphate pathway was involved in glucose catabolism. 10.1074/jbc.M401232200}, author = {Roger, Olivier and Lavigne, Regis and Mahmoud, Mohamed and Buisson, Corinne and Onno, Bernard and Zhang, Ben-Li and Robins, Richard J.}, citeulike-article-id = {2374184}, citeulike-linkout-0 = {http://dx.doi.org/10.1074/jbc.M401232200}, citeulike-linkout-1 = {http://www.jbc.org/cgi/content/abstract/279/24/24923}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/15051719}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=15051719}, day = {11}, doi = {10.1074/jbc.M401232200}, journal = {J. Biol. Chem.}, keywords = {abundance, acid, bacteria, fermentation, glucose, lab, lactic, natural, nmr, pathway}, month = {June}, number = {24}, pages = {24923--24928}, posted-at = {2008-02-14 14:19:35}, priority = {0}, title = {Quantitative 2H NMR at Natural Abundance Can Distinguish the Pathway Used for Glucose Fermentation by Lactic Acid Bacteria}, url = {http://dx.doi.org/10.1074/jbc.M401232200}, volume = {279}, year = {2004} }

TY - JOUR ID - citeulike:2374184 L3 - citeulike-article-id:2374184 N2 - For any given metabolic pathway, isotope redistribution coefficients (aij) that characterize the specific derivation of each hydrogen atom can be defined. By using quantitative deuterium NMR, the redistribution of deuterium at natural abundance in lactic acid produced by the bacterial fermentation of glucose has been determined for each non-labile hydrogen atom of glucose or water and the hydrogen atoms of lactic acid. Distinct differences are observed in the lactic acid isolated from Lactococcus lactis and Leuconostoc mesenteroides that can be interpreted in terms of the different fermentative pathways used. Specifically, the affiliations observed between the H1, H3, and H4 positions of glucose with methyl and hydroxymethylene of lactic acid can give quantitative information on whether the glycolytic or the reductive pentose-phosphate pathway was involved in glucose catabolism. 10.1074/jbc.M401232200 IS - 24 JF - J. Biol. Chem. EP - 24928 TI - Quantitative 2H NMR at Natural Abundance Can Distinguish the Pathway Used for Glucose Fermentation by Lactic Acid Bacteria VL - 279 SP - 24923 KW - abundance KW - acid KW - bacteria KW - fermentation KW - glucose KW - lab KW - lactic KW - natural KW - nmr KW - pathway AU - Roger, Olivier AU - Lavigne, Regis AU - Mahmoud, Mohamed AU - Buisson, Corinne AU - Onno, Bernard AU - Zhang, Ben-Li AU - Robins, Richard PY - 2004/06/11/ UR - http://dx.doi.org/10.1074/jbc.M401232200 ER -