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Hemin Reconstitutes Proton Extrusion in an H+-ATPase-Negative Mutant of Lactococcus lactis Export

by: Lars M. Blank, Brian J. Koebmann, Ole Michelsen, Lars K. Nielsen, Peter R. Jensen
J. Bacteriol., Vol. 183, No. 22. (15 November 2001), pp. 6707-6709.

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atpase hemin lactis lactococcus proton_extrusion

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H+-ATPase is considered essential for growth of Lactococcus lactis. However, media containing hemin restored the aerobic growth of an H+-ATPase-negative mutant, suggesting that hemin complements proton extrusion. We show that inverted membrane vesicles prepared from hemin-grown L. lactis cells are capable of coupling NADH oxidation to proton translocation. 10.1128/JB.183.22.6707-6709.2001


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